Spectral imaging toolbox: segmentation, hyperstack reconstruction, and batch processing of spectral images for the determination of cell and model membrane lipid order
<h4>Background</h4> <p>Spectral imaging with polarity-sensitive fluorescent probes enables the quantification of cell and model membrane physical properties, including local hydration, fluidity, and lateral lipid packing, usually characterized by the generalized polarization (GP)...
Main Authors: | Aron, M, Browning, R, Carugo, D, Sezgin, E, Bernardino de la Serna, J, Eggeling, C, Stride, E |
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Format: | Journal article |
Published: |
BioMed Central
2017
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