Combined biochemical and cytological analysis of membrane trafficking using lectins.
We have tested the application of high-mannose-binding lectins as analytical reagents to identify N-glycans in the early secretory pathway of HeLa cells during subcellular fractionation and cytochemistry. Post-endoplasmic reticulum (ER) pre-Golgi intermediates were separated from the ER on Nycodenz-...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2013
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