Activity-based ubiquitin-specific protease (USP) profiling of virus-infected and malignant human cells.
The family of ubiquitin (Ub)-specific proteases (USP) removes Ub from Ub conjugates and regulates a variety of cellular processes. The human genome contains many putative USP-encoding genes, but little is known about USP tissue distribution, pattern of expression, activity, and substrate specificity...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
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2004
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| _version_ | 1797089205751382016 |
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| author | Ovaa, H Kessler, B Rolén, U Galardy, P Ploegh, H Masucci, MG |
| author_facet | Ovaa, H Kessler, B Rolén, U Galardy, P Ploegh, H Masucci, MG |
| author_sort | Ovaa, H |
| collection | OXFORD |
| description | The family of ubiquitin (Ub)-specific proteases (USP) removes Ub from Ub conjugates and regulates a variety of cellular processes. The human genome contains many putative USP-encoding genes, but little is known about USP tissue distribution, pattern of expression, activity, and substrate specificity. We have used a chemistry-based functional proteomics approach to identify active USPs in normal, virus-infected, and tumor-derived human cells. Depending on tissue origin and stage of activation/differentiation, different USP activity profiles were revealed. The activity of specific USPs, including USP5, -7, -9, -13, -15, and -22, was up-regulated by mitogen activation or virus infection in normal T and B lymphocytes. UCH-L1 was highly expressed in tumor cell lines of epithelial and hematopoietic cell origin but was not detected in freshly isolated and mitogen-activated cells. Up-regulation of this USP was a late event in the establishment of Epstein-Barr virus-immortalized lymphoblastoid cell lines and correlated with enhanced proliferation, suggesting a possible role in growth transformation. |
| first_indexed | 2024-03-07T03:01:03Z |
| format | Journal article |
| id | oxford-uuid:b0f99ef5-cc68-431b-885a-b0c4b4cb6bd0 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:01:03Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:b0f99ef5-cc68-431b-885a-b0c4b4cb6bd02022-03-27T04:00:26ZActivity-based ubiquitin-specific protease (USP) profiling of virus-infected and malignant human cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:b0f99ef5-cc68-431b-885a-b0c4b4cb6bd0EnglishSymplectic Elements at Oxford2004Ovaa, HKessler, BRolén, UGalardy, PPloegh, HMasucci, MGThe family of ubiquitin (Ub)-specific proteases (USP) removes Ub from Ub conjugates and regulates a variety of cellular processes. The human genome contains many putative USP-encoding genes, but little is known about USP tissue distribution, pattern of expression, activity, and substrate specificity. We have used a chemistry-based functional proteomics approach to identify active USPs in normal, virus-infected, and tumor-derived human cells. Depending on tissue origin and stage of activation/differentiation, different USP activity profiles were revealed. The activity of specific USPs, including USP5, -7, -9, -13, -15, and -22, was up-regulated by mitogen activation or virus infection in normal T and B lymphocytes. UCH-L1 was highly expressed in tumor cell lines of epithelial and hematopoietic cell origin but was not detected in freshly isolated and mitogen-activated cells. Up-regulation of this USP was a late event in the establishment of Epstein-Barr virus-immortalized lymphoblastoid cell lines and correlated with enhanced proliferation, suggesting a possible role in growth transformation. |
| spellingShingle | Ovaa, H Kessler, B Rolén, U Galardy, P Ploegh, H Masucci, MG Activity-based ubiquitin-specific protease (USP) profiling of virus-infected and malignant human cells. |
| title | Activity-based ubiquitin-specific protease (USP) profiling of virus-infected and malignant human cells. |
| title_full | Activity-based ubiquitin-specific protease (USP) profiling of virus-infected and malignant human cells. |
| title_fullStr | Activity-based ubiquitin-specific protease (USP) profiling of virus-infected and malignant human cells. |
| title_full_unstemmed | Activity-based ubiquitin-specific protease (USP) profiling of virus-infected and malignant human cells. |
| title_short | Activity-based ubiquitin-specific protease (USP) profiling of virus-infected and malignant human cells. |
| title_sort | activity based ubiquitin specific protease usp profiling of virus infected and malignant human cells |
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