Protein targeting of an unusual, evolutionarily conserved adenylate kinase to a eukaryotic flagellum
The eukaryotic flagellum is a large structure into which specific constituent proteins must be targeted, transported and assembled after their synthesis in the cytoplasm. Using Trypanosoma brucei and a proteomic approach, we have identified and characterized a novel set of adenylate kinase proteins...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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American Society for Cell Biology
2004
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| _version_ | 1826291632193929216 |
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| author | Pullen, T Ginger, M Gaskell, S Gull, K |
| author_facet | Pullen, T Ginger, M Gaskell, S Gull, K |
| author_sort | Pullen, T |
| collection | OXFORD |
| description | The eukaryotic flagellum is a large structure into which specific constituent proteins must be targeted, transported and assembled after their synthesis in the cytoplasm. Using Trypanosoma brucei and a proteomic approach, we have identified and characterized a novel set of adenylate kinase proteins that are localized to the flagellum. These proteins represent unique isoforms that are targeted to the flagellum by an N-terminal extension to the protein and are incorporated into an extraaxonemal structure (the paraflagellar rod). We show that the N-terminal extension is both necessary for isoform location in the flagellum and sufficient for targeting of a green fluorescent protein reporter protein to the flagellum. Moreover, these N-terminal extension sequences are conserved in evolution and we find that they allow the identification of novel adenylate kinases in the genomes of humans and worms. Given the existence of specific isoforms of certain metabolic enzymes, and targeting sequences for these isoforms, we suggest that these isoforms form part of a complex, "solid-phase" metabolic capability that is built into the eukaryotic flagellum. |
| first_indexed | 2024-03-07T03:02:19Z |
| format | Journal article |
| id | oxford-uuid:b15dcc01-578b-4e57-bbb1-711171040b3d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:02:19Z |
| publishDate | 2004 |
| publisher | American Society for Cell Biology |
| record_format | dspace |
| spelling | oxford-uuid:b15dcc01-578b-4e57-bbb1-711171040b3d2022-03-27T04:03:32ZProtein targeting of an unusual, evolutionarily conserved adenylate kinase to a eukaryotic flagellumJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:b15dcc01-578b-4e57-bbb1-711171040b3dChemistry & allied sciencesPathologyEnglishOxford University Research Archive - ValetAmerican Society for Cell Biology2004Pullen, TGinger, MGaskell, SGull, KThe eukaryotic flagellum is a large structure into which specific constituent proteins must be targeted, transported and assembled after their synthesis in the cytoplasm. Using Trypanosoma brucei and a proteomic approach, we have identified and characterized a novel set of adenylate kinase proteins that are localized to the flagellum. These proteins represent unique isoforms that are targeted to the flagellum by an N-terminal extension to the protein and are incorporated into an extraaxonemal structure (the paraflagellar rod). We show that the N-terminal extension is both necessary for isoform location in the flagellum and sufficient for targeting of a green fluorescent protein reporter protein to the flagellum. Moreover, these N-terminal extension sequences are conserved in evolution and we find that they allow the identification of novel adenylate kinases in the genomes of humans and worms. Given the existence of specific isoforms of certain metabolic enzymes, and targeting sequences for these isoforms, we suggest that these isoforms form part of a complex, "solid-phase" metabolic capability that is built into the eukaryotic flagellum. |
| spellingShingle | Chemistry & allied sciences Pathology Pullen, T Ginger, M Gaskell, S Gull, K Protein targeting of an unusual, evolutionarily conserved adenylate kinase to a eukaryotic flagellum |
| title | Protein targeting of an unusual, evolutionarily conserved adenylate kinase to a eukaryotic flagellum |
| title_full | Protein targeting of an unusual, evolutionarily conserved adenylate kinase to a eukaryotic flagellum |
| title_fullStr | Protein targeting of an unusual, evolutionarily conserved adenylate kinase to a eukaryotic flagellum |
| title_full_unstemmed | Protein targeting of an unusual, evolutionarily conserved adenylate kinase to a eukaryotic flagellum |
| title_short | Protein targeting of an unusual, evolutionarily conserved adenylate kinase to a eukaryotic flagellum |
| title_sort | protein targeting of an unusual evolutionarily conserved adenylate kinase to a eukaryotic flagellum |
| topic | Chemistry & allied sciences Pathology |
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