Elevated intracellular cyclic‐di‐GMP level in Shewanella oneidensis increases expression of c‐type cytochromes

Electrochemically active biofilms are capable of exchanging electrons with solid electron acceptors and have many energy and environmental applications such as bioelectricity generation and environmental remediation. The performance of electrochemically active biofilms is usually dependent on c‐type...

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Main Authors: Ng, CK, Xu, J, Cai, Z, Yang, L, Thompson, IP, Huang, WE, Cao, B
Format: Journal article
Language:English
Published: Wiley 2020
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author Ng, CK
Xu, J
Cai, Z
Yang, L
Thompson, IP
Huang, WE
Cao, B
author_facet Ng, CK
Xu, J
Cai, Z
Yang, L
Thompson, IP
Huang, WE
Cao, B
author_sort Ng, CK
collection OXFORD
description Electrochemically active biofilms are capable of exchanging electrons with solid electron acceptors and have many energy and environmental applications such as bioelectricity generation and environmental remediation. The performance of electrochemically active biofilms is usually dependent on c‐type cytochromes, while biofilm development is controlled by a signal cascade mediated by the intracellular secondary messenger bis‐(3ʹ‐5ʹ) cyclic dimeric guanosine monophosphate (c‐di‐GMP). However, it is unclear whether there are any links between the c‐di‐GMP regulatory system and the expression of c‐type cytochromes. In this study, we constructed a S. oneidensis MR‐1 strain with a higher cytoplasmic c‐di‐GMP level by constitutively expressing a c‐di‐GMP synthase and it exhibited expected c‐di‐GMP‐influenced traits, such as lowered motility and increased biofilm formation. Compared to MR‐1 wild‐type strain, the high c‐di‐GMP strain had a higher Fe(III) reduction rate (21.58 vs 11.88 pM of Fe(III)/h cell) and greater expression of genes that code for the proteins involved in the Mtr pathway, including CymA, MtrA, MtrB, MtrC and OmcA. Furthermore, single‐cell Raman microspectroscopy (SCRM) revealed a great increase of c‐type cytochromes in the high c‐di‐GMP strain as compared to MR‐1 wild‐type strain. Our results reveal for the first time that the c‐di‐GMP regulation system indirectly or directly positively regulates the expression of cytochromes involved in the extracellular electron transport (EET) in S. oneidensis, which would help to understand the regulatory mechanism of c‐di‐GMP on electricity production in bacteria.
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spelling oxford-uuid:b1668e2a-75e9-40c0-b79e-11e0278f6c122022-03-27T04:03:53ZElevated intracellular cyclic‐di‐GMP level in Shewanella oneidensis increases expression of c‐type cytochromesJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:b1668e2a-75e9-40c0-b79e-11e0278f6c12EnglishSymplectic ElementsWiley2020Ng, CKXu, JCai, ZYang, LThompson, IPHuang, WECao, BElectrochemically active biofilms are capable of exchanging electrons with solid electron acceptors and have many energy and environmental applications such as bioelectricity generation and environmental remediation. The performance of electrochemically active biofilms is usually dependent on c‐type cytochromes, while biofilm development is controlled by a signal cascade mediated by the intracellular secondary messenger bis‐(3ʹ‐5ʹ) cyclic dimeric guanosine monophosphate (c‐di‐GMP). However, it is unclear whether there are any links between the c‐di‐GMP regulatory system and the expression of c‐type cytochromes. In this study, we constructed a S. oneidensis MR‐1 strain with a higher cytoplasmic c‐di‐GMP level by constitutively expressing a c‐di‐GMP synthase and it exhibited expected c‐di‐GMP‐influenced traits, such as lowered motility and increased biofilm formation. Compared to MR‐1 wild‐type strain, the high c‐di‐GMP strain had a higher Fe(III) reduction rate (21.58 vs 11.88 pM of Fe(III)/h cell) and greater expression of genes that code for the proteins involved in the Mtr pathway, including CymA, MtrA, MtrB, MtrC and OmcA. Furthermore, single‐cell Raman microspectroscopy (SCRM) revealed a great increase of c‐type cytochromes in the high c‐di‐GMP strain as compared to MR‐1 wild‐type strain. Our results reveal for the first time that the c‐di‐GMP regulation system indirectly or directly positively regulates the expression of cytochromes involved in the extracellular electron transport (EET) in S. oneidensis, which would help to understand the regulatory mechanism of c‐di‐GMP on electricity production in bacteria.
spellingShingle Ng, CK
Xu, J
Cai, Z
Yang, L
Thompson, IP
Huang, WE
Cao, B
Elevated intracellular cyclic‐di‐GMP level in Shewanella oneidensis increases expression of c‐type cytochromes
title Elevated intracellular cyclic‐di‐GMP level in Shewanella oneidensis increases expression of c‐type cytochromes
title_full Elevated intracellular cyclic‐di‐GMP level in Shewanella oneidensis increases expression of c‐type cytochromes
title_fullStr Elevated intracellular cyclic‐di‐GMP level in Shewanella oneidensis increases expression of c‐type cytochromes
title_full_unstemmed Elevated intracellular cyclic‐di‐GMP level in Shewanella oneidensis increases expression of c‐type cytochromes
title_short Elevated intracellular cyclic‐di‐GMP level in Shewanella oneidensis increases expression of c‐type cytochromes
title_sort elevated intracellular cyclic di gmp level in shewanella oneidensis increases expression of c type cytochromes
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