Enhanced non-vitreous cryopreservation of immortalized and primary cells by ice-growth inhibiting polymers.
Cell cryopreservation is an essential tool in modern biotechnology and medicine. The ability to freeze, store and distribute materials underpins basic cell biology and enables storage of donor cells needed for transplantation and regenerative medicine. However, many cell types do not survive freezin...
| Hlavní autoři: | , , , , |
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| Médium: | Journal article |
| Jazyk: | English |
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Royal Society of Chemistry
2016
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| _version_ | 1826291685828591616 |
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| author | Deller, R Pessin, J Vatish, M Mitchell, D Gibson, M |
| author_facet | Deller, R Pessin, J Vatish, M Mitchell, D Gibson, M |
| author_sort | Deller, R |
| collection | OXFORD |
| description | Cell cryopreservation is an essential tool in modern biotechnology and medicine. The ability to freeze, store and distribute materials underpins basic cell biology and enables storage of donor cells needed for transplantation and regenerative medicine. However, many cell types do not survive freezing and the current state-of-the-art involves the addition of significant amounts of organic solvents as cryoprotectants, which themselves can be cytotoxic, or simply interfere with assays. A key cause of cell death in cryopreservation is ice recrystallization (growth), which primarily occurs during thawing. Here it is demonstrated that the addition of ice recrystalization inhibiting polymers to solutions containing low (non vitrifying) concentrations of DMSO enhance cell recovery rates by up to 75%. Cell functionality is also demonstrated using a placental cell line, and enhanced cryopreservation of primary rat hepatocytes is additionally shown. The crucial role of the polymers architecture (chain length) is shown, with shorter polymers being more effective than longer ones. |
| first_indexed | 2024-03-07T03:03:08Z |
| format | Journal article |
| id | oxford-uuid:b19ed957-e33e-4c37-a48e-3ad44c4b6a37 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:03:08Z |
| publishDate | 2016 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | oxford-uuid:b19ed957-e33e-4c37-a48e-3ad44c4b6a372022-03-27T04:05:31ZEnhanced non-vitreous cryopreservation of immortalized and primary cells by ice-growth inhibiting polymers.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:b19ed957-e33e-4c37-a48e-3ad44c4b6a37EnglishSymplectic Elements at OxfordRoyal Society of Chemistry2016Deller, RPessin, JVatish, MMitchell, DGibson, MCell cryopreservation is an essential tool in modern biotechnology and medicine. The ability to freeze, store and distribute materials underpins basic cell biology and enables storage of donor cells needed for transplantation and regenerative medicine. However, many cell types do not survive freezing and the current state-of-the-art involves the addition of significant amounts of organic solvents as cryoprotectants, which themselves can be cytotoxic, or simply interfere with assays. A key cause of cell death in cryopreservation is ice recrystallization (growth), which primarily occurs during thawing. Here it is demonstrated that the addition of ice recrystalization inhibiting polymers to solutions containing low (non vitrifying) concentrations of DMSO enhance cell recovery rates by up to 75%. Cell functionality is also demonstrated using a placental cell line, and enhanced cryopreservation of primary rat hepatocytes is additionally shown. The crucial role of the polymers architecture (chain length) is shown, with shorter polymers being more effective than longer ones. |
| spellingShingle | Deller, R Pessin, J Vatish, M Mitchell, D Gibson, M Enhanced non-vitreous cryopreservation of immortalized and primary cells by ice-growth inhibiting polymers. |
| title | Enhanced non-vitreous cryopreservation of immortalized and primary cells by ice-growth inhibiting polymers. |
| title_full | Enhanced non-vitreous cryopreservation of immortalized and primary cells by ice-growth inhibiting polymers. |
| title_fullStr | Enhanced non-vitreous cryopreservation of immortalized and primary cells by ice-growth inhibiting polymers. |
| title_full_unstemmed | Enhanced non-vitreous cryopreservation of immortalized and primary cells by ice-growth inhibiting polymers. |
| title_short | Enhanced non-vitreous cryopreservation of immortalized and primary cells by ice-growth inhibiting polymers. |
| title_sort | enhanced non vitreous cryopreservation of immortalized and primary cells by ice growth inhibiting polymers |
| work_keys_str_mv | AT dellerr enhancednonvitreouscryopreservationofimmortalizedandprimarycellsbyicegrowthinhibitingpolymers AT pessinj enhancednonvitreouscryopreservationofimmortalizedandprimarycellsbyicegrowthinhibitingpolymers AT vatishm enhancednonvitreouscryopreservationofimmortalizedandprimarycellsbyicegrowthinhibitingpolymers AT mitchelld enhancednonvitreouscryopreservationofimmortalizedandprimarycellsbyicegrowthinhibitingpolymers AT gibsonm enhancednonvitreouscryopreservationofimmortalizedandprimarycellsbyicegrowthinhibitingpolymers |