Overexpression of LC3A autophagy protein in follicular and diffuse large B-cell lymphomas.
BACKGROUND AND OBJECTIVES: Autophagy is a self-degradation mechanism induced under stress conditions in all eukaryotic cells. Its activity in human lymphomas has not been studied as yet. METHODS: In this study, the autophagic activity of lymphoid cells was investigated in follicular lymphomas (FL;...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
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2013
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| _version_ | 1797089385222504448 |
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| author | Giatromanolaki, A Koukourakis, M Pouliliou, S Gatter, K Pezzella, F Harris, A Sivridis, E |
| author_facet | Giatromanolaki, A Koukourakis, M Pouliliou, S Gatter, K Pezzella, F Harris, A Sivridis, E |
| author_sort | Giatromanolaki, A |
| collection | OXFORD |
| description | BACKGROUND AND OBJECTIVES: Autophagy is a self-degradation mechanism induced under stress conditions in all eukaryotic cells. Its activity in human lymphomas has not been studied as yet. METHODS: In this study, the autophagic activity of lymphoid cells was investigated in follicular lymphomas (FL; 48 cases), diffuse large B-cell lymphomas (DLBCL; 78 cases), and in reactive follicular hyperplasias (41 cases), using the light chain 3A (LC3A) antibody and a standard immunohistochemical technique. RESULTS: In all cases, the pattern of LC3A reactivity was uniformly diffuse cytoplasmic, but expressed more frequently in FLs (68.8%) than in DLBCLs (41%) (p=0.02), and much more commonly in DLBCLs than in reactive lymph nodes (24.3%) (p<0.006). Interestingly, FLs expressing LC3A in >10% of lymphoid cells (high reactivity) were associated with the hypoxia-related protein HIF1α and the enzyme of anaerobic metabolism lactate dehydrogenase LDH5 (p=0.004 and p=0.003, respectively). Such associations, however, were not a feature in DLBCLs of increased LC3A activity. CONCLUSIONS: LC3A expression in FLs is hypoxia-induced, whereas its expression in DLBCLs may be regulated by other molecular mechanisms. The current study provides a tool for further assessment of autophagic activity in translational and autophagy targeting therapy studies. |
| first_indexed | 2024-03-07T03:03:24Z |
| format | Journal article |
| id | oxford-uuid:b1b63943-f9f4-4bbf-8fd1-ae97f84d9b65 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:03:24Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | oxford-uuid:b1b63943-f9f4-4bbf-8fd1-ae97f84d9b652022-03-27T04:06:04ZOverexpression of LC3A autophagy protein in follicular and diffuse large B-cell lymphomas.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:b1b63943-f9f4-4bbf-8fd1-ae97f84d9b65EnglishSymplectic Elements at Oxford2013Giatromanolaki, AKoukourakis, MPouliliou, SGatter, KPezzella, FHarris, ASivridis, E BACKGROUND AND OBJECTIVES: Autophagy is a self-degradation mechanism induced under stress conditions in all eukaryotic cells. Its activity in human lymphomas has not been studied as yet. METHODS: In this study, the autophagic activity of lymphoid cells was investigated in follicular lymphomas (FL; 48 cases), diffuse large B-cell lymphomas (DLBCL; 78 cases), and in reactive follicular hyperplasias (41 cases), using the light chain 3A (LC3A) antibody and a standard immunohistochemical technique. RESULTS: In all cases, the pattern of LC3A reactivity was uniformly diffuse cytoplasmic, but expressed more frequently in FLs (68.8%) than in DLBCLs (41%) (p=0.02), and much more commonly in DLBCLs than in reactive lymph nodes (24.3%) (p<0.006). Interestingly, FLs expressing LC3A in >10% of lymphoid cells (high reactivity) were associated with the hypoxia-related protein HIF1α and the enzyme of anaerobic metabolism lactate dehydrogenase LDH5 (p=0.004 and p=0.003, respectively). Such associations, however, were not a feature in DLBCLs of increased LC3A activity. CONCLUSIONS: LC3A expression in FLs is hypoxia-induced, whereas its expression in DLBCLs may be regulated by other molecular mechanisms. The current study provides a tool for further assessment of autophagic activity in translational and autophagy targeting therapy studies. |
| spellingShingle | Giatromanolaki, A Koukourakis, M Pouliliou, S Gatter, K Pezzella, F Harris, A Sivridis, E Overexpression of LC3A autophagy protein in follicular and diffuse large B-cell lymphomas. |
| title | Overexpression of LC3A autophagy protein in follicular and diffuse large B-cell lymphomas. |
| title_full | Overexpression of LC3A autophagy protein in follicular and diffuse large B-cell lymphomas. |
| title_fullStr | Overexpression of LC3A autophagy protein in follicular and diffuse large B-cell lymphomas. |
| title_full_unstemmed | Overexpression of LC3A autophagy protein in follicular and diffuse large B-cell lymphomas. |
| title_short | Overexpression of LC3A autophagy protein in follicular and diffuse large B-cell lymphomas. |
| title_sort | overexpression of lc3a autophagy protein in follicular and diffuse large b cell lymphomas |
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