Hypoxia-activated small molecule-induced gene expression
Hypoxia, a condition of reduced oxygen, occurs in a wide variety of biological contexts, including solid tumors and bacterial biofilms, which are relevant to human health. Consequently, the development of chemical tools to study hypoxia is vital. Here we report a hypoxia-activated, small-molecule-me...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
American Chemical Society
2018
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| _version_ | 1797089429628649472 |
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| author | Collins, S Saha, J Bouchez, L Hammond, E Conway, S |
| author_facet | Collins, S Saha, J Bouchez, L Hammond, E Conway, S |
| author_sort | Collins, S |
| collection | OXFORD |
| description | Hypoxia, a condition of reduced oxygen, occurs in a wide variety of biological contexts, including solid tumors and bacterial biofilms, which are relevant to human health. Consequently, the development of chemical tools to study hypoxia is vital. Here we report a hypoxia-activated, small-molecule-mediated gene expression system using a bioreductive prodrug of the inducer isopropyl 1-thio-β-d-galactopyranoside. As a proof-of-concept we have placed the production of a green fluorescent protein under the control of hypoxia. Our system has the potential to be extended to regulate the production of any given protein of choice. |
| first_indexed | 2024-03-07T03:04:02Z |
| format | Journal article |
| id | oxford-uuid:b1e88f04-750e-4110-968a-a71aca0dbbaf |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:04:02Z |
| publishDate | 2018 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | oxford-uuid:b1e88f04-750e-4110-968a-a71aca0dbbaf2022-03-27T04:07:42ZHypoxia-activated small molecule-induced gene expressionJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:b1e88f04-750e-4110-968a-a71aca0dbbafEnglishSymplectic Elements at OxfordAmerican Chemical Society2018Collins, SSaha, JBouchez, LHammond, EConway, SHypoxia, a condition of reduced oxygen, occurs in a wide variety of biological contexts, including solid tumors and bacterial biofilms, which are relevant to human health. Consequently, the development of chemical tools to study hypoxia is vital. Here we report a hypoxia-activated, small-molecule-mediated gene expression system using a bioreductive prodrug of the inducer isopropyl 1-thio-β-d-galactopyranoside. As a proof-of-concept we have placed the production of a green fluorescent protein under the control of hypoxia. Our system has the potential to be extended to regulate the production of any given protein of choice. |
| spellingShingle | Collins, S Saha, J Bouchez, L Hammond, E Conway, S Hypoxia-activated small molecule-induced gene expression |
| title | Hypoxia-activated small molecule-induced gene expression |
| title_full | Hypoxia-activated small molecule-induced gene expression |
| title_fullStr | Hypoxia-activated small molecule-induced gene expression |
| title_full_unstemmed | Hypoxia-activated small molecule-induced gene expression |
| title_short | Hypoxia-activated small molecule-induced gene expression |
| title_sort | hypoxia activated small molecule induced gene expression |
| work_keys_str_mv | AT collinss hypoxiaactivatedsmallmoleculeinducedgeneexpression AT sahaj hypoxiaactivatedsmallmoleculeinducedgeneexpression AT bouchezl hypoxiaactivatedsmallmoleculeinducedgeneexpression AT hammonde hypoxiaactivatedsmallmoleculeinducedgeneexpression AT conways hypoxiaactivatedsmallmoleculeinducedgeneexpression |