Efficient propagation and cloning of human T cells in the absence of antigen by means of OKT3, interleukin 2, and antigen-presenting cells.
The analysis of T lymphocytes infiltrating tissues afflicted by autoimmune diseases may provide major clues towards understanding the pathogenesis of such diseases. Currently the best approach to studying heterogeneous populations such as T lymphocytes involves long-term culture and cloning. In orde...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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1988
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| _version_ | 1797089493373681664 |
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| author | Londei, M Grubeck-Loebenstein, B de Berardinis, P Greenall, C Feldmann, M |
| author_facet | Londei, M Grubeck-Loebenstein, B de Berardinis, P Greenall, C Feldmann, M |
| author_sort | Londei, M |
| collection | OXFORD |
| description | The analysis of T lymphocytes infiltrating tissues afflicted by autoimmune diseases may provide major clues towards understanding the pathogenesis of such diseases. Currently the best approach to studying heterogeneous populations such as T lymphocytes involves long-term culture and cloning. In order to grow and clone T lymphocytes, regular restimulation with the specific antigen is essential, otherwise growth will stop and/or specificity may be lost. In autoimmune diseases the antigens involved in triggering the immunological reaction of T cells are usually unknown. Therefore an alternative way of stimulating T lymphocytes without loss of specificity is clearly needed. Here we describe the cloning and expansion of antigen-specific T cell clones from the blood of a healthy donor to sizeable numbers of cells (greater than 10(8)) by means of anti-CD3 monoclonal antibody and recombinant IL-2. The results obtained showed that this approach can be used to clone and 'expand' T lymphocytes that retain antigen specificity over a prolonged period, in this case over 10 weeks. This technique has been used to clone and expand T lymphocytes infiltrating the affected tissues in a variety of autoimmune disorders such as Hashimoto's thyroiditis, Graves' disease, and rheumatoid arthritis, and is an efficient method of propagating T cells, by mimicking the antigenic stimulus. |
| first_indexed | 2024-03-07T03:04:53Z |
| format | Journal article |
| id | oxford-uuid:b228389f-9352-4445-9896-fb35feff9c40 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:04:53Z |
| publishDate | 1988 |
| record_format | dspace |
| spelling | oxford-uuid:b228389f-9352-4445-9896-fb35feff9c402022-03-27T04:09:48ZEfficient propagation and cloning of human T cells in the absence of antigen by means of OKT3, interleukin 2, and antigen-presenting cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:b228389f-9352-4445-9896-fb35feff9c40EnglishSymplectic Elements at Oxford1988Londei, MGrubeck-Loebenstein, Bde Berardinis, PGreenall, CFeldmann, MThe analysis of T lymphocytes infiltrating tissues afflicted by autoimmune diseases may provide major clues towards understanding the pathogenesis of such diseases. Currently the best approach to studying heterogeneous populations such as T lymphocytes involves long-term culture and cloning. In order to grow and clone T lymphocytes, regular restimulation with the specific antigen is essential, otherwise growth will stop and/or specificity may be lost. In autoimmune diseases the antigens involved in triggering the immunological reaction of T cells are usually unknown. Therefore an alternative way of stimulating T lymphocytes without loss of specificity is clearly needed. Here we describe the cloning and expansion of antigen-specific T cell clones from the blood of a healthy donor to sizeable numbers of cells (greater than 10(8)) by means of anti-CD3 monoclonal antibody and recombinant IL-2. The results obtained showed that this approach can be used to clone and 'expand' T lymphocytes that retain antigen specificity over a prolonged period, in this case over 10 weeks. This technique has been used to clone and expand T lymphocytes infiltrating the affected tissues in a variety of autoimmune disorders such as Hashimoto's thyroiditis, Graves' disease, and rheumatoid arthritis, and is an efficient method of propagating T cells, by mimicking the antigenic stimulus. |
| spellingShingle | Londei, M Grubeck-Loebenstein, B de Berardinis, P Greenall, C Feldmann, M Efficient propagation and cloning of human T cells in the absence of antigen by means of OKT3, interleukin 2, and antigen-presenting cells. |
| title | Efficient propagation and cloning of human T cells in the absence of antigen by means of OKT3, interleukin 2, and antigen-presenting cells. |
| title_full | Efficient propagation and cloning of human T cells in the absence of antigen by means of OKT3, interleukin 2, and antigen-presenting cells. |
| title_fullStr | Efficient propagation and cloning of human T cells in the absence of antigen by means of OKT3, interleukin 2, and antigen-presenting cells. |
| title_full_unstemmed | Efficient propagation and cloning of human T cells in the absence of antigen by means of OKT3, interleukin 2, and antigen-presenting cells. |
| title_short | Efficient propagation and cloning of human T cells in the absence of antigen by means of OKT3, interleukin 2, and antigen-presenting cells. |
| title_sort | efficient propagation and cloning of human t cells in the absence of antigen by means of okt3 interleukin 2 and antigen presenting cells |
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