Disassembly of intact multiprotein complexes in the gas phase.

The observation of multiprotein complexes by mass spectrometry formerly relied upon chemical cross-linking to maintain interactions. Recent technological developments have enabled the observation of intact macromolecular complexes without modification. These assemblies, with masses far in excess of...

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Những tác giả chính: Rostom, A, Robinson, C
Định dạng: Journal article
Ngôn ngữ:English
Được phát hành: 1999
Miêu tả
Tóm tắt:The observation of multiprotein complexes by mass spectrometry formerly relied upon chemical cross-linking to maintain interactions. Recent technological developments have enabled the observation of intact macromolecular complexes without modification. These assemblies, with masses far in excess of those measured previously, can be examined through controlled dissociation in the mass spectrometer, revealing information about their subunit interactions and topology.