A mycobacterial growth inhibition assay (MGIA) for bovine TB vaccine development

Human tuberculosis remains a significant cause of mortality and morbidity throughout the world. The global economic impact of bovine TB is considerable. An effective vaccine would be the most cost-effective way to control both epidemics, particularly in emerging economies. TB vaccine research would...

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मुख्य लेखकों: Pepponi, I, Khatri, B, Tanner, R, Villarreal-Ramos, B, Vordermeier, M, McShane, H
स्वरूप: Journal article
प्रकाशित: Elsevier 2017
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author Pepponi, I
Khatri, B
Tanner, R
Villarreal-Ramos, B
Vordermeier, M
McShane, H
author_facet Pepponi, I
Khatri, B
Tanner, R
Villarreal-Ramos, B
Vordermeier, M
McShane, H
author_sort Pepponi, I
collection OXFORD
description Human tuberculosis remains a significant cause of mortality and morbidity throughout the world. The global economic impact of bovine TB is considerable. An effective vaccine would be the most cost-effective way to control both epidemics, particularly in emerging economies. TB vaccine research would benefit from the identification of an immune correlate of protection with which vaccines could be gated at both preclinical and clinical levels. In-vitro mycobacterial growth inhibition assays (MGIA) are functional assays that include most aspects of the complex host immune response to mycobacteria, and they may serve as functional immune correlates for vaccine development. We applied to cattle an MGIA that was developed for use with human and murine samples. Several technical difficulties were encountered while transferring it to the cattle model. However, our data demonstrate that the assay was not discriminatory in cattle and further work is needed before using it for bovine TB vaccine development.
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spelling oxford-uuid:b442b18d-7f9c-4bff-9569-5c0f75e7a0c22022-03-27T04:24:47ZA mycobacterial growth inhibition assay (MGIA) for bovine TB vaccine developmentJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:b442b18d-7f9c-4bff-9569-5c0f75e7a0c2Symplectic Elements at OxfordElsevier2017Pepponi, IKhatri, BTanner, RVillarreal-Ramos, BVordermeier, MMcShane, HHuman tuberculosis remains a significant cause of mortality and morbidity throughout the world. The global economic impact of bovine TB is considerable. An effective vaccine would be the most cost-effective way to control both epidemics, particularly in emerging economies. TB vaccine research would benefit from the identification of an immune correlate of protection with which vaccines could be gated at both preclinical and clinical levels. In-vitro mycobacterial growth inhibition assays (MGIA) are functional assays that include most aspects of the complex host immune response to mycobacteria, and they may serve as functional immune correlates for vaccine development. We applied to cattle an MGIA that was developed for use with human and murine samples. Several technical difficulties were encountered while transferring it to the cattle model. However, our data demonstrate that the assay was not discriminatory in cattle and further work is needed before using it for bovine TB vaccine development.
spellingShingle Pepponi, I
Khatri, B
Tanner, R
Villarreal-Ramos, B
Vordermeier, M
McShane, H
A mycobacterial growth inhibition assay (MGIA) for bovine TB vaccine development
title A mycobacterial growth inhibition assay (MGIA) for bovine TB vaccine development
title_full A mycobacterial growth inhibition assay (MGIA) for bovine TB vaccine development
title_fullStr A mycobacterial growth inhibition assay (MGIA) for bovine TB vaccine development
title_full_unstemmed A mycobacterial growth inhibition assay (MGIA) for bovine TB vaccine development
title_short A mycobacterial growth inhibition assay (MGIA) for bovine TB vaccine development
title_sort mycobacterial growth inhibition assay mgia for bovine tb vaccine development
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