Circulating platelet-derived and placenta-derived microparticles expose Flt-1 in preeclampsia.
BACKGROUND: Flt-1 is secreted by various cells and elevated concentrations are present in preeclampsia affecting vascular function. Microparticles from these cells may expose Flt-1. We evaluated whether Flt-1 is microparticle-associated in preeclampsia, and established the origin of Flt-1-exposing...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2008
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| _version_ | 1826292267872157696 |
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| author | Lok, C Böing, A Sargent, I Sooranna, SR van der Post, J Nieuwland, R Sturk, A |
| author_facet | Lok, C Böing, A Sargent, I Sooranna, SR van der Post, J Nieuwland, R Sturk, A |
| author_sort | Lok, C |
| collection | OXFORD |
| description | BACKGROUND: Flt-1 is secreted by various cells and elevated concentrations are present in preeclampsia affecting vascular function. Microparticles from these cells may expose Flt-1. We evaluated whether Flt-1 is microparticle-associated in preeclampsia, and established the origin of Flt-1-exposing microparticles. METHODS: The concentration of Flt-1 was measured in samples from preeclamptic patients, pregnant and nonpregnant women by enzyme-linked immunosorbent assay. Microparticles were analyzed by flow cytometry. Western blot determined the different forms of Flt-1. RESULTS: Noncell bound Flt-1 was elevated in preeclampsia compared to controls. A fraction (5%) was associated with microparticles in preeclampsia. Flt-1-exposing microparticles were increased in preeclampsia compared to normotensive pregnancy (p = 0.02). Full-length Flt-1, was identified in microparticles of platelet and placental origin. CONCLUSION: Full-length Flt-1 is associated with platelet and placenta-derived microparticles. Possibly, the presentation of Flt-1 on the membrane of a microparticle might alter its function, particularly if it acts in synergism with other exposed vasoactive molecules. |
| first_indexed | 2024-03-07T03:12:03Z |
| format | Journal article |
| id | oxford-uuid:b48694d5-2f31-4c9d-8e2d-65bffd514e67 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:12:03Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:b48694d5-2f31-4c9d-8e2d-65bffd514e672022-03-27T04:26:44ZCirculating platelet-derived and placenta-derived microparticles expose Flt-1 in preeclampsia.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:b48694d5-2f31-4c9d-8e2d-65bffd514e67EnglishSymplectic Elements at Oxford2008Lok, CBöing, ASargent, ISooranna, SRvan der Post, JNieuwland, RSturk, A BACKGROUND: Flt-1 is secreted by various cells and elevated concentrations are present in preeclampsia affecting vascular function. Microparticles from these cells may expose Flt-1. We evaluated whether Flt-1 is microparticle-associated in preeclampsia, and established the origin of Flt-1-exposing microparticles. METHODS: The concentration of Flt-1 was measured in samples from preeclamptic patients, pregnant and nonpregnant women by enzyme-linked immunosorbent assay. Microparticles were analyzed by flow cytometry. Western blot determined the different forms of Flt-1. RESULTS: Noncell bound Flt-1 was elevated in preeclampsia compared to controls. A fraction (5%) was associated with microparticles in preeclampsia. Flt-1-exposing microparticles were increased in preeclampsia compared to normotensive pregnancy (p = 0.02). Full-length Flt-1, was identified in microparticles of platelet and placental origin. CONCLUSION: Full-length Flt-1 is associated with platelet and placenta-derived microparticles. Possibly, the presentation of Flt-1 on the membrane of a microparticle might alter its function, particularly if it acts in synergism with other exposed vasoactive molecules. |
| spellingShingle | Lok, C Böing, A Sargent, I Sooranna, SR van der Post, J Nieuwland, R Sturk, A Circulating platelet-derived and placenta-derived microparticles expose Flt-1 in preeclampsia. |
| title | Circulating platelet-derived and placenta-derived microparticles expose Flt-1 in preeclampsia. |
| title_full | Circulating platelet-derived and placenta-derived microparticles expose Flt-1 in preeclampsia. |
| title_fullStr | Circulating platelet-derived and placenta-derived microparticles expose Flt-1 in preeclampsia. |
| title_full_unstemmed | Circulating platelet-derived and placenta-derived microparticles expose Flt-1 in preeclampsia. |
| title_short | Circulating platelet-derived and placenta-derived microparticles expose Flt-1 in preeclampsia. |
| title_sort | circulating platelet derived and placenta derived microparticles expose flt 1 in preeclampsia |
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