Expression of functional granulocyte colony-stimulating factor receptors on human B-lymphocytic leukemia cells.
We analyzed the expression of cell surface antigens and granulocyte colony-stimulating factor (G-CSF) receptors using flow cytometry, the expression of G-CSF mRNA receptor, using reverse transcription (RT)-PCR, and tested the effect of G-CSF on leukemia colony formation. A total of 14 lymphocytic le...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2000
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| _version_ | 1826292317010526208 |
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| author | Handa, A Kashimura, T Takeuchi, S Yamamoto, A Murohashi, I Bessho, M Hirashima, K |
| author_facet | Handa, A Kashimura, T Takeuchi, S Yamamoto, A Murohashi, I Bessho, M Hirashima, K |
| author_sort | Handa, A |
| collection | OXFORD |
| description | We analyzed the expression of cell surface antigens and granulocyte colony-stimulating factor (G-CSF) receptors using flow cytometry, the expression of G-CSF mRNA receptor, using reverse transcription (RT)-PCR, and tested the effect of G-CSF on leukemia colony formation. A total of 14 lymphocytic leukemia patients were examined, seven with acute lymphocytic leukemia (ALL), two with adult T-cell leukemia (ATL), two with B-chronic lymphocytic leukemia (CLL), two with chronic myelocytic leukemia in lymphoid blastic crisis (CML-LBC), and one with plasma cell leukemia (PCL). The presence of G-CSF receptors was demonstrated in 4/14 (29%) patients, two with ALL, one with CLL, and one with CML-LBC, and was associated with stimulation of leukemia clonogenic cell growth by G-CSF. In addition, all four positive leukemia cell types expressed typical B-cell antigens. Our results indicated that G-CSF receptors are expressed on some portion of B-lymphoid leukemia and that their receptors are functional as growth stimulators. |
| first_indexed | 2024-03-07T03:12:49Z |
| format | Journal article |
| id | oxford-uuid:b4c81f7a-96d4-49a0-90fa-a079d7a77d62 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:12:49Z |
| publishDate | 2000 |
| record_format | dspace |
| spelling | oxford-uuid:b4c81f7a-96d4-49a0-90fa-a079d7a77d622022-03-27T04:28:36ZExpression of functional granulocyte colony-stimulating factor receptors on human B-lymphocytic leukemia cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:b4c81f7a-96d4-49a0-90fa-a079d7a77d62EnglishSymplectic Elements at Oxford2000Handa, AKashimura, TTakeuchi, SYamamoto, AMurohashi, IBessho, MHirashima, KWe analyzed the expression of cell surface antigens and granulocyte colony-stimulating factor (G-CSF) receptors using flow cytometry, the expression of G-CSF mRNA receptor, using reverse transcription (RT)-PCR, and tested the effect of G-CSF on leukemia colony formation. A total of 14 lymphocytic leukemia patients were examined, seven with acute lymphocytic leukemia (ALL), two with adult T-cell leukemia (ATL), two with B-chronic lymphocytic leukemia (CLL), two with chronic myelocytic leukemia in lymphoid blastic crisis (CML-LBC), and one with plasma cell leukemia (PCL). The presence of G-CSF receptors was demonstrated in 4/14 (29%) patients, two with ALL, one with CLL, and one with CML-LBC, and was associated with stimulation of leukemia clonogenic cell growth by G-CSF. In addition, all four positive leukemia cell types expressed typical B-cell antigens. Our results indicated that G-CSF receptors are expressed on some portion of B-lymphoid leukemia and that their receptors are functional as growth stimulators. |
| spellingShingle | Handa, A Kashimura, T Takeuchi, S Yamamoto, A Murohashi, I Bessho, M Hirashima, K Expression of functional granulocyte colony-stimulating factor receptors on human B-lymphocytic leukemia cells. |
| title | Expression of functional granulocyte colony-stimulating factor receptors on human B-lymphocytic leukemia cells. |
| title_full | Expression of functional granulocyte colony-stimulating factor receptors on human B-lymphocytic leukemia cells. |
| title_fullStr | Expression of functional granulocyte colony-stimulating factor receptors on human B-lymphocytic leukemia cells. |
| title_full_unstemmed | Expression of functional granulocyte colony-stimulating factor receptors on human B-lymphocytic leukemia cells. |
| title_short | Expression of functional granulocyte colony-stimulating factor receptors on human B-lymphocytic leukemia cells. |
| title_sort | expression of functional granulocyte colony stimulating factor receptors on human b lymphocytic leukemia cells |
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