Fully efficient chromosome dimer resolution in Escherichia coli cells lacking the integral membrane domain of FtsK.
In bacteria, septum formation frequently initiates before the last steps of chromosome segregation. This is notably the case when chromosome dimers are formed by homologous recombination. Chromosome segregation then requires the activity of a double-stranded DNA transporter anchored at the septum by...
| Glavni autori: | , |
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| Format: | Journal article |
| Jezik: | English |
| Izdano: |
2010
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| _version_ | 1826292428587401216 |
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| author | Dubarry, N Barre, F |
| author_facet | Dubarry, N Barre, F |
| author_sort | Dubarry, N |
| collection | OXFORD |
| description | In bacteria, septum formation frequently initiates before the last steps of chromosome segregation. This is notably the case when chromosome dimers are formed by homologous recombination. Chromosome segregation then requires the activity of a double-stranded DNA transporter anchored at the septum by an integral membrane domain, FtsK. It was proposed that the transmembrane segments of proteins of the FtsK family form pores across lipid bilayers for the transport of DNA. Here, we show that truncated Escherichia coli FtsK proteins lacking all of the FtsK transmembrane segments allow for the efficient resolution of chromosome dimers if they are connected to a septal targeting peptide through a sufficiently long linker. These results indicate that FtsK does not need to transport DNA through a pore formed by its integral membrane domain. We propose therefore that FtsK transports DNA before membrane fusion, at a time when there is still an opening in the constricted septum. |
| first_indexed | 2024-03-07T03:14:32Z |
| format | Journal article |
| id | oxford-uuid:b5580a68-eadf-4eff-8e50-975b1ada346a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:14:32Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oxford-uuid:b5580a68-eadf-4eff-8e50-975b1ada346a2022-03-27T04:32:43ZFully efficient chromosome dimer resolution in Escherichia coli cells lacking the integral membrane domain of FtsK.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:b5580a68-eadf-4eff-8e50-975b1ada346aEnglishSymplectic Elements at Oxford2010Dubarry, NBarre, FIn bacteria, septum formation frequently initiates before the last steps of chromosome segregation. This is notably the case when chromosome dimers are formed by homologous recombination. Chromosome segregation then requires the activity of a double-stranded DNA transporter anchored at the septum by an integral membrane domain, FtsK. It was proposed that the transmembrane segments of proteins of the FtsK family form pores across lipid bilayers for the transport of DNA. Here, we show that truncated Escherichia coli FtsK proteins lacking all of the FtsK transmembrane segments allow for the efficient resolution of chromosome dimers if they are connected to a septal targeting peptide through a sufficiently long linker. These results indicate that FtsK does not need to transport DNA through a pore formed by its integral membrane domain. We propose therefore that FtsK transports DNA before membrane fusion, at a time when there is still an opening in the constricted septum. |
| spellingShingle | Dubarry, N Barre, F Fully efficient chromosome dimer resolution in Escherichia coli cells lacking the integral membrane domain of FtsK. |
| title | Fully efficient chromosome dimer resolution in Escherichia coli cells lacking the integral membrane domain of FtsK. |
| title_full | Fully efficient chromosome dimer resolution in Escherichia coli cells lacking the integral membrane domain of FtsK. |
| title_fullStr | Fully efficient chromosome dimer resolution in Escherichia coli cells lacking the integral membrane domain of FtsK. |
| title_full_unstemmed | Fully efficient chromosome dimer resolution in Escherichia coli cells lacking the integral membrane domain of FtsK. |
| title_short | Fully efficient chromosome dimer resolution in Escherichia coli cells lacking the integral membrane domain of FtsK. |
| title_sort | fully efficient chromosome dimer resolution in escherichia coli cells lacking the integral membrane domain of ftsk |
| work_keys_str_mv | AT dubarryn fullyefficientchromosomedimerresolutioninescherichiacolicellslackingtheintegralmembranedomainofftsk AT barref fullyefficientchromosomedimerresolutioninescherichiacolicellslackingtheintegralmembranedomainofftsk |