Distribution of class 1 integrons in historic and contemporary collections of human pathogenic Escherichia coli
Integrons play a major role in the evolution and spread of antimicrobial resistance in human pathogens, including Escherichia coli. This study describes the occurrence of class 1 integrons in human pathogenic E. coli, in three isolate collections involving three periods from the last 100 years (i) t...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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Public Library of Science
2020
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| _version_ | 1797090163292110848 |
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| author | Maiden, M Suetterlin, S Bray, J Tano, E |
| author_facet | Maiden, M Suetterlin, S Bray, J Tano, E |
| author_sort | Maiden, M |
| collection | OXFORD |
| description | Integrons play a major role in the evolution and spread of antimicrobial resistance in human pathogens, including Escherichia coli. This study describes the occurrence of class 1 integrons in human pathogenic E. coli, in three isolate collections involving three periods from the last 100 years (i) the Murray collection (n = 58 bacteria isolated from the 1910s to 1940s); (ii) the E. coli reference (ECOR) collection (n = 37 isolates mainly from the 1980s); and (iii) a recently assembled collection (n = 88 isolates obtained in 2016). High-quality whole genome sequences (WGSs) were available for all isolates. Integrons were detected in the WGSs with the program IntegronFinder and the results compared with three established methods: (i) polymerase chain reaction detection of the integrase gene; (ii) BLAST searching using draft genomes; and (iii) mapping of short reads. No integrons were found in any of the Murray Collection isolates; however, integrons were present in 3% of the isolates from ECOR collection, assembled in the 1980s, and 26% of the isolates from the 2010s. Similarly, antimicrobial resistance determinants were not present in the Murray Collection isolates, whereas they were present in 19% of the ECOR Collection isolates and in 55% of the isolates obtained in during the 2010s. |
| first_indexed | 2024-03-07T03:14:34Z |
| format | Journal article |
| id | oxford-uuid:b55990bb-5589-4ba7-88e9-3c9f70a9b57a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:14:34Z |
| publishDate | 2020 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | oxford-uuid:b55990bb-5589-4ba7-88e9-3c9f70a9b57a2022-03-27T04:32:50ZDistribution of class 1 integrons in historic and contemporary collections of human pathogenic Escherichia coliJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:b55990bb-5589-4ba7-88e9-3c9f70a9b57aEnglishSymplectic ElementsPublic Library of Science2020Maiden, MSuetterlin, SBray, JTano, EIntegrons play a major role in the evolution and spread of antimicrobial resistance in human pathogens, including Escherichia coli. This study describes the occurrence of class 1 integrons in human pathogenic E. coli, in three isolate collections involving three periods from the last 100 years (i) the Murray collection (n = 58 bacteria isolated from the 1910s to 1940s); (ii) the E. coli reference (ECOR) collection (n = 37 isolates mainly from the 1980s); and (iii) a recently assembled collection (n = 88 isolates obtained in 2016). High-quality whole genome sequences (WGSs) were available for all isolates. Integrons were detected in the WGSs with the program IntegronFinder and the results compared with three established methods: (i) polymerase chain reaction detection of the integrase gene; (ii) BLAST searching using draft genomes; and (iii) mapping of short reads. No integrons were found in any of the Murray Collection isolates; however, integrons were present in 3% of the isolates from ECOR collection, assembled in the 1980s, and 26% of the isolates from the 2010s. Similarly, antimicrobial resistance determinants were not present in the Murray Collection isolates, whereas they were present in 19% of the ECOR Collection isolates and in 55% of the isolates obtained in during the 2010s. |
| spellingShingle | Maiden, M Suetterlin, S Bray, J Tano, E Distribution of class 1 integrons in historic and contemporary collections of human pathogenic Escherichia coli |
| title | Distribution of class 1 integrons in historic and contemporary collections of human pathogenic Escherichia coli |
| title_full | Distribution of class 1 integrons in historic and contemporary collections of human pathogenic Escherichia coli |
| title_fullStr | Distribution of class 1 integrons in historic and contemporary collections of human pathogenic Escherichia coli |
| title_full_unstemmed | Distribution of class 1 integrons in historic and contemporary collections of human pathogenic Escherichia coli |
| title_short | Distribution of class 1 integrons in historic and contemporary collections of human pathogenic Escherichia coli |
| title_sort | distribution of class 1 integrons in historic and contemporary collections of human pathogenic escherichia coli |
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