Study of MAIT cell activation in viral infections in vivo
MAIT cells are abundant, highly evolutionarily conserved innate-like lymphocytes expressing a semi-invariant T cell receptor (TCR), which recognizes microbially derived small intermediate molecules from the riboflavin biosynthetic pathway. However, in addition to their TCR-mediated functions they ca...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Humana Press
2019
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| _version_ | 1797090223620882432 |
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| author | Hinks, TSC van Wilgenburg, B Wang, H Loh, L Koutsakos, M Kedzierska, K Corbett, AJ Chen, Z |
| author_facet | Hinks, TSC van Wilgenburg, B Wang, H Loh, L Koutsakos, M Kedzierska, K Corbett, AJ Chen, Z |
| author_sort | Hinks, TSC |
| collection | OXFORD |
| description | MAIT cells are abundant, highly evolutionarily conserved innate-like lymphocytes expressing a semi-invariant T cell receptor (TCR), which recognizes microbially derived small intermediate molecules from the riboflavin biosynthetic pathway. However, in addition to their TCR-mediated functions they can also be activated in a TCR-independent manner via cytokines including IL-12, -15, -18, and type I interferon. Emerging data suggest that they are expanded and activated by a range of viral infections, and significantly that they can contribute to a protective anti-viral response. Here we describe methods used to investigate these anti-viral functions in vivo in murine models. To overcome the technical challenge that MAIT cells are rare in specific pathogen-free laboratory mice, we describe how pulmonary MAIT cells can be expanded using intranasal bacterial infection or a combination of synthetic MAIT cell antigen and TLR agonists. We also describe protocols for adoptive transfer of MAIT cells, methods for lung homogenization for plaque assays, and surface and intracellular cytokine staining to determine MAIT cell activation. |
| first_indexed | 2024-03-07T03:15:25Z |
| format | Journal article |
| id | oxford-uuid:b59d838d-fb27-4342-9ee1-14173684f541 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:15:25Z |
| publishDate | 2019 |
| publisher | Humana Press |
| record_format | dspace |
| spelling | oxford-uuid:b59d838d-fb27-4342-9ee1-14173684f5412022-03-27T04:34:54ZStudy of MAIT cell activation in viral infections in vivoJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:b59d838d-fb27-4342-9ee1-14173684f541EnglishSymplectic ElementsHumana Press2019Hinks, TSCvan Wilgenburg, BWang, HLoh, LKoutsakos, MKedzierska, KCorbett, AJChen, ZMAIT cells are abundant, highly evolutionarily conserved innate-like lymphocytes expressing a semi-invariant T cell receptor (TCR), which recognizes microbially derived small intermediate molecules from the riboflavin biosynthetic pathway. However, in addition to their TCR-mediated functions they can also be activated in a TCR-independent manner via cytokines including IL-12, -15, -18, and type I interferon. Emerging data suggest that they are expanded and activated by a range of viral infections, and significantly that they can contribute to a protective anti-viral response. Here we describe methods used to investigate these anti-viral functions in vivo in murine models. To overcome the technical challenge that MAIT cells are rare in specific pathogen-free laboratory mice, we describe how pulmonary MAIT cells can be expanded using intranasal bacterial infection or a combination of synthetic MAIT cell antigen and TLR agonists. We also describe protocols for adoptive transfer of MAIT cells, methods for lung homogenization for plaque assays, and surface and intracellular cytokine staining to determine MAIT cell activation. |
| spellingShingle | Hinks, TSC van Wilgenburg, B Wang, H Loh, L Koutsakos, M Kedzierska, K Corbett, AJ Chen, Z Study of MAIT cell activation in viral infections in vivo |
| title | Study of MAIT cell activation in viral infections in vivo |
| title_full | Study of MAIT cell activation in viral infections in vivo |
| title_fullStr | Study of MAIT cell activation in viral infections in vivo |
| title_full_unstemmed | Study of MAIT cell activation in viral infections in vivo |
| title_short | Study of MAIT cell activation in viral infections in vivo |
| title_sort | study of mait cell activation in viral infections in vivo |
| work_keys_str_mv | AT hinkstsc studyofmaitcellactivationinviralinfectionsinvivo AT vanwilgenburgb studyofmaitcellactivationinviralinfectionsinvivo AT wangh studyofmaitcellactivationinviralinfectionsinvivo AT lohl studyofmaitcellactivationinviralinfectionsinvivo AT koutsakosm studyofmaitcellactivationinviralinfectionsinvivo AT kedzierskak studyofmaitcellactivationinviralinfectionsinvivo AT corbettaj studyofmaitcellactivationinviralinfectionsinvivo AT chenz studyofmaitcellactivationinviralinfectionsinvivo |