Quantitative study of protein-protein interactions in live cell by dual-color fluorescence correlation spectroscopy.

Quantitative study of protein-protein interactions in live cell by dual-color fluorescence correlation spectroscopy.

Dual-color FCS is a powerful method to monitor protein-protein interactions in living cells. The main idea is based on the cross-correlation analysis of temporal fluorescence intensity fluctuations of two fluorescent proteins to obtain their co-diffusion and relative concentration. But, when perform...

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Bibliografiske detaljer
Main Authors:	Padilla-Parra, S, Audugé, N, Coppey-Moisan, M, Tramier, M
Format:	Book section
Udgivet:	Humana Press Inc. 2014

Lignende værker

Dual-color fluorescence lifetime correlation spectroscopy to quantify protein-protein interactions in live cell.
af: Padilla-Parra, S, et al.
Udgivet: (2011)

Non fitting based FRET-FLIM analysis approaches applied to quantify protein-protein interactions in live cells
af: Padilla-Parra, S, et al.
Udgivet: (2011)

Non fitting based FRET-FLIM analysis approaches applied to quantify protein-protein interactions in live cells
af: Padilla-Parra, S, et al.
Udgivet: (2011)

Quantitative comparison of different fluorescent protein couples for fast FRET-FLIM acquisition.
af: Padilla-Parra, S, et al.
Udgivet: (2009)

Quantitative FRET analysis by fast acquisition time domain FLIM at high spatial resolution in living cells.
af: Padilla-Parra, S, et al.
Udgivet: (2008)

Chromatin condensation fluctuations rather than steady-state predict chromatin accessibility
af: Audugé, N, et al.
Udgivet: (2019)

Dual-color total internal reflection fluorescence cross-correlation spectroscopy.
af: Leutenegger, M, et al.
Udgivet: (2006)

FRET microscopy in the living cell: different approaches, strengths and weaknesses.
af: Padilla-Parra, S, et al.
Udgivet: (2012)

Deubiquitination Detection of p53 Protein in Living Cells by Fluorescence Cross-Correlation Spectroscopy
af: Yaoqi Liu, et al.
Udgivet: (2023-09-01)

Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy.
af: Andresen, M, et al.
Udgivet: (2008)

Characterization of protein adsorption onto FePt nanoparticles using dual-focus fluorescence correlation spectroscopy
af: Pauline Maffre, et al.
Udgivet: (2011-07-01)

Vectors for multi-color bimolecular fluorescence complementation to investigate protein-protein interactions in living plant cells
af: Kuang Lin-Yun, et al.
Udgivet: (2008-10-01)

Three-Color Imaging Using Fluorescent Proteins in Living Zebrafish Embryos
af: Kenneth R. Finley, et al.
Udgivet: (2001-07-01)

Simple and Efficient Detection Scheme of Two-Color Fluorescence Correlation Spectroscopy for Protein Dynamics Investigation from Nanoseconds to Milliseconds
af: Yutaka Sano, et al.
Udgivet: (2023-11-01)

Fluorescence microscopy data for quantitative mobility and interaction analysis of proteins in living cells
af: Veerle Lemmens, et al.
Udgivet: (2020-04-01)

A new approach to dual-color two-photon microscopy with fluorescent proteins
af: Rebane Aleks, et al.
Udgivet: (2010-02-01)

Characterization of Fluorescent Proteins for Three- and Four-Color Live-Cell Imaging in S. cerevisiae.
af: Ryo Higuchi-Sanabria, et al.
Udgivet: (2016-01-01)

Dual-probe fluorescence spectroscopy for sensitive quantitation of Alzheimer’s amyloid pathology
af: Anastasiia A. Stepanchuk, et al.
Udgivet: (2022-10-01)

SAM domain-based protein oligomerization observed by live-cell fluorescence fluctuation spectroscopy.
af: Brian D Slaughter, et al.
Udgivet: (2008-04-01)

Dynamic interaction of amphiphysin with N-WASP regulates actin assembly.
af: Yamada, H, et al.
Udgivet: (2009)

Dual-Color Bioluminescence Analysis for Quantitatively Monitoring G-Protein-Coupled Receptor and β-Arrestin Interactions
af: Naomi Misawa, et al.
Udgivet: (2011-02-01)

Fluorescence Correlation Spectroscopy Reveals Efficient Cytosolic Delivery of Protein Cargo by Cell-Permeant Miniature Proteins
af: Rebecca F. Wissner, et al.
Udgivet: (2018-09-01)

Biophysical Characterization of Membrane Proteins Embedded in Nanodiscs Using Fluorescence Correlation Spectroscopy
af: Matthew J. Laurence, et al.
Udgivet: (2022-03-01)

Bayesian Model Selection Applied to the Analysis of Fluorescence Correlation Spectroscopy Data of Fluorescent Proteins in Vitro and in Vivo
af: Sun, Guangyu, et al.
Udgivet: (2015)

Interaction between Spike Protein of SARS-CoV-2 and Human Virus Receptor ACE2 Using Two-Color Fluorescence Cross-Correlation Spectroscopy
af: Ai Fujimoto, et al.
Udgivet: (2021-11-01)

Multiplexing PKA and ERK1&2 kinases FRET biosensors in living cells using single excitation wavelength dual colour FLIM
af: Demeautis, C, et al.
Udgivet: (2017)

A Quantitative Study of Internal and External Interactions of Homodimeric Glucocorticoid Receptor Using Fluorescence Cross-Correlation Spectroscopy in a Live Cell
af: Manisha Tiwari, et al.
Udgivet: (2017-06-01)

Protocol for evaluating drug-protein interactions based on fluorescence spectroscopy
af: Elaheh Jalali, et al.
Udgivet: (2024-12-01)

Fluorescent quantitation method for differentiating the nativity of green fluorescent protein
af: Chew, Few Ne, et al.
Udgivet: (2011)

Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging
af: Hela Benaissa, et al.
Udgivet: (2021-11-01)

Colorful protein-based fluorescent probes for collagen imaging.
af: Stijn J A Aper, et al.
Udgivet: (2014-01-01)

A new protocol for fluorescent quantitative labeling of individual proteins for live cell internalization assays
af: Alche Juan De Dios, et al.
Udgivet: (2024-01-01)

Interaction between Astaxanthin and Whey Protein Based on Fluorescence and Ultraviolet Spectroscopy
af: Yingyuan ZHAO, et al.
Udgivet: (2022-01-01)

An alternative framework for fluorescence correlation spectroscopy
af: Sina Jazani, et al.
Udgivet: (2019-08-01)

Liquid-microjet photoelectron spectroscopy of the green fluorescent protein chromophore
af: Omri Tau, et al.
Udgivet: (2022-01-01)

Detecting protein aggregation and interactions in live cells: a guide to Number and Brightness
af: Nolan, R, et al.
Udgivet: (2017)

Two-Color Green Fluorescent Protein Time-Lapse Imaging
af: Jan Ellenberg, et al.
Udgivet: (1998-11-01)

"Color Timer" mice: visualization of neuronal differentiation with fluorescent proteins
af: Kanki Hiroaki, et al.
Udgivet: (2010-02-01)

Monitoring the Interaction between Thermally Induced Whey Protein and Anthocyanin by Fluorescence Quenching Spectroscopy
af: Shuai Ren, et al.
Udgivet: (2021-02-01)

827Spatio-Temporal Quantification of FRET in living cells by fast time-domain FLIM: a comparative study of non-fitting methods
af: Leray, A, et al.
Udgivet: (2013)