Quantitative study of protein-protein interactions in live cell by dual-color fluorescence correlation spectroscopy.
Dual-color FCS is a powerful method to monitor protein-protein interactions in living cells. The main idea is based on the cross-correlation analysis of temporal fluorescence intensity fluctuations of two fluorescent proteins to obtain their co-diffusion and relative concentration. But, when perform...
| Main Authors: | , , , |
|---|---|
| Format: | Book section |
| Udgivet: |
Humana Press Inc.
2014
|