Quantitative study of protein-protein interactions in live cell by dual-color fluorescence correlation spectroscopy.
Dual-color FCS is a powerful method to monitor protein-protein interactions in living cells. The main idea is based on the cross-correlation analysis of temporal fluorescence intensity fluctuations of two fluorescent proteins to obtain their co-diffusion and relative concentration. But, when perform...
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| 格式: | Book section |
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Humana Press Inc.
2014
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