Adult thymic medullary epithelium is maintained and regenerated by linage-restricted cells rather than bipotent progenitors
Medullary thymic epithelial cells (mTECs) play an essential role in establishing self-tolerance in T cells. mTECs originate from bipotent TEC progenitors that generate both mTECs and cortical TECs (cTECs), although mTEC-restricted progenitors also have been reported. Here, we report in vivo fate-map...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
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Cell Press
2015
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| _version_ | 1826307333229117440 |
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| author | Ohigashi, I Zuklys, S Sakata, M Mayer, C Hamazaki, Y Minato, N Hollander, G Takahama, Y |
| author_facet | Ohigashi, I Zuklys, S Sakata, M Mayer, C Hamazaki, Y Minato, N Hollander, G Takahama, Y |
| author_sort | Ohigashi, I |
| collection | OXFORD |
| description | Medullary thymic epithelial cells (mTECs) play an essential role in establishing self-tolerance in T cells. mTECs originate from bipotent TEC progenitors that generate both mTECs and cortical TECs (cTECs), although mTEC-restricted progenitors also have been reported. Here, we report in vivo fate-mapping analysis of cells that transcribe β5t, a cTEC trait expressed in bipotent progenitors, during a given period in mice. We show that, in adult mice, most mTECs are derived from progenitors that transcribe β5t during embryogenesis and the neonatal period up to 1 week of age. The contribution of adult β5t+ progenitors was minor even during injury-triggered regeneration. Our results further demonstrate that adult mTEC-restricted progenitors are derived from perinatal β5t+ progenitors. These results indicate that the adult thymic medullary epithelium is maintained and regenerated by mTEC-lineage cells that pass beyond the bipotent stage during early ontogeny. |
| first_indexed | 2024-03-07T07:01:38Z |
| format | Journal article |
| id | oxford-uuid:b66b036b-34b5-4032-9ecb-3897a9db8fcb |
| institution | University of Oxford |
| last_indexed | 2024-03-07T07:01:38Z |
| publishDate | 2015 |
| publisher | Cell Press |
| record_format | dspace |
| spelling | oxford-uuid:b66b036b-34b5-4032-9ecb-3897a9db8fcb2022-03-29T17:18:47ZAdult thymic medullary epithelium is maintained and regenerated by linage-restricted cells rather than bipotent progenitorsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:b66b036b-34b5-4032-9ecb-3897a9db8fcbORA DepositCell Press2015Ohigashi, IZuklys, SSakata, MMayer, CHamazaki, YMinato, NHollander, GTakahama, YMedullary thymic epithelial cells (mTECs) play an essential role in establishing self-tolerance in T cells. mTECs originate from bipotent TEC progenitors that generate both mTECs and cortical TECs (cTECs), although mTEC-restricted progenitors also have been reported. Here, we report in vivo fate-mapping analysis of cells that transcribe β5t, a cTEC trait expressed in bipotent progenitors, during a given period in mice. We show that, in adult mice, most mTECs are derived from progenitors that transcribe β5t during embryogenesis and the neonatal period up to 1 week of age. The contribution of adult β5t+ progenitors was minor even during injury-triggered regeneration. Our results further demonstrate that adult mTEC-restricted progenitors are derived from perinatal β5t+ progenitors. These results indicate that the adult thymic medullary epithelium is maintained and regenerated by mTEC-lineage cells that pass beyond the bipotent stage during early ontogeny. |
| spellingShingle | Ohigashi, I Zuklys, S Sakata, M Mayer, C Hamazaki, Y Minato, N Hollander, G Takahama, Y Adult thymic medullary epithelium is maintained and regenerated by linage-restricted cells rather than bipotent progenitors |
| title | Adult thymic medullary epithelium is maintained and regenerated by linage-restricted cells rather than bipotent progenitors |
| title_full | Adult thymic medullary epithelium is maintained and regenerated by linage-restricted cells rather than bipotent progenitors |
| title_fullStr | Adult thymic medullary epithelium is maintained and regenerated by linage-restricted cells rather than bipotent progenitors |
| title_full_unstemmed | Adult thymic medullary epithelium is maintained and regenerated by linage-restricted cells rather than bipotent progenitors |
| title_short | Adult thymic medullary epithelium is maintained and regenerated by linage-restricted cells rather than bipotent progenitors |
| title_sort | adult thymic medullary epithelium is maintained and regenerated by linage restricted cells rather than bipotent progenitors |
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