Electron spin resonance study of light-induced conformational changes in nitroxide labelled bovine rhodopsin.
Bovine rhodopsin was isolated in the unbleached form as a retinal disc membrane suspension and spin-labelled with 4-maleimido-2,2,6,6-tetramethylpiperidine-N-oxyl. Both conventional and saturation transfer electron spin resonance methods were used to investigate the sensitivity of the spin-label to...
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| Format: | Journal article |
| Language: | English |
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1988
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| _version_ | 1826292653748125696 |
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| author | Rowntree, J Watts, A |
| author_facet | Rowntree, J Watts, A |
| author_sort | Rowntree, J |
| collection | OXFORD |
| description | Bovine rhodopsin was isolated in the unbleached form as a retinal disc membrane suspension and spin-labelled with 4-maleimido-2,2,6,6-tetramethylpiperidine-N-oxyl. Both conventional and saturation transfer electron spin resonance methods were used to investigate the sensitivity of the spin-label to conformational changes of rhodopsin induced by both transient and long-term exposure to light. The results indicate that the ESR methods do display sensitivity to such changes. An exponential decay curve with a time constant of 10 s was obtained by following the height of a single peak in the saturation transfer electron spin resonance spectrum in response to a single light flash. |
| first_indexed | 2024-03-07T03:18:02Z |
| format | Journal article |
| id | oxford-uuid:b6786552-1661-4ab0-9587-a30c4c9cb060 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:18:02Z |
| publishDate | 1988 |
| record_format | dspace |
| spelling | oxford-uuid:b6786552-1661-4ab0-9587-a30c4c9cb0602022-03-27T04:41:10ZElectron spin resonance study of light-induced conformational changes in nitroxide labelled bovine rhodopsin.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:b6786552-1661-4ab0-9587-a30c4c9cb060EnglishSymplectic Elements at Oxford1988Rowntree, JWatts, ABovine rhodopsin was isolated in the unbleached form as a retinal disc membrane suspension and spin-labelled with 4-maleimido-2,2,6,6-tetramethylpiperidine-N-oxyl. Both conventional and saturation transfer electron spin resonance methods were used to investigate the sensitivity of the spin-label to conformational changes of rhodopsin induced by both transient and long-term exposure to light. The results indicate that the ESR methods do display sensitivity to such changes. An exponential decay curve with a time constant of 10 s was obtained by following the height of a single peak in the saturation transfer electron spin resonance spectrum in response to a single light flash. |
| spellingShingle | Rowntree, J Watts, A Electron spin resonance study of light-induced conformational changes in nitroxide labelled bovine rhodopsin. |
| title | Electron spin resonance study of light-induced conformational changes in nitroxide labelled bovine rhodopsin. |
| title_full | Electron spin resonance study of light-induced conformational changes in nitroxide labelled bovine rhodopsin. |
| title_fullStr | Electron spin resonance study of light-induced conformational changes in nitroxide labelled bovine rhodopsin. |
| title_full_unstemmed | Electron spin resonance study of light-induced conformational changes in nitroxide labelled bovine rhodopsin. |
| title_short | Electron spin resonance study of light-induced conformational changes in nitroxide labelled bovine rhodopsin. |
| title_sort | electron spin resonance study of light induced conformational changes in nitroxide labelled bovine rhodopsin |
| work_keys_str_mv | AT rowntreej electronspinresonancestudyoflightinducedconformationalchangesinnitroxidelabelledbovinerhodopsin AT wattsa electronspinresonancestudyoflightinducedconformationalchangesinnitroxidelabelledbovinerhodopsin |