Rapid formation of non-native contacts during the folding of HPr revealed by real-time photo-CIDNP NMR and stopped-flow fluorescence experiments.
We report the combined use of real-time photo-CIDNP NMR and stopped-flow fluorescence techniques to study the kinetic refolding of a set of mutants of a small globular protein, HPr, in which each of the four phenylalanine residues has in turn been replaced by a tryptophan residue. The results indica...
Glavni autori: | Canet, D, Lyon, C, Scheek, R, Robillard, G, Dobson, C, Hore, P, van Nuland, N |
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Format: | Journal article |
Jezik: | English |
Izdano: |
2003
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