Evaluation of ovotransferrin-matrix metal-affinity metalloprotein chromatography for Pu(IV) separations
Metal-Affinity metalloprotein chromatography has been suggested to have potential for selectively removing actinides from solution. To evaluate this potential, characterization of Pu(IV) and Fe(III) binding to an ovotransferrin-Sepharos™ 4B matrix, with oxalate and carbonate synergistic anions, was...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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2003
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| _version_ | 1797090576790716416 |
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| author | Myers, W Ruggiero, C Reilly, S Neu, M |
| author_facet | Myers, W Ruggiero, C Reilly, S Neu, M |
| author_sort | Myers, W |
| collection | OXFORD |
| description | Metal-Affinity metalloprotein chromatography has been suggested to have potential for selectively removing actinides from solution. To evaluate this potential, characterization of Pu(IV) and Fe(III) binding to an ovotransferrin-Sepharos™ 4B matrix, with oxalate and carbonate synergistic anions, was performed. Metal binding capacity was determined for metal concentrations ranging from 2 × 10-6 to 1 × 10 -3 M Fe(III) and 2.9 × 10-5 to 2.6 × 10 -4 M Pu(IV), where both metals were added as a solution of 10:1 nitrilotriacetic acid:metal. Metal binding capacity was also determined over the pH range 3.0 to 9.2. The metal binding properties of the immobilized ovotransferrin were found to differ from those reported for free-protein studies. Distribution coefficients were low: for metal solution concentrations of 0.02, 0.04, 0.10, and 0.20 mM, coefficients were 540, 180, 23, and 12mL/g for Fe(III) and 20, 12, 7.2, and 6.0 mL/g for Pu(IV), respectively. The capacity of the material was less dependent on pH than expected, varying by only two μmoles metal/g matrix for both Fe(III) and Pu(IV) over the pH range of 4.2 to 9.5. This study illustrates limitations of metalloprotein chromatography for Pu(IV) separation. |
| first_indexed | 2024-03-07T03:20:38Z |
| format | Journal article |
| id | oxford-uuid:b74a8e9a-8daa-4ae8-8b9a-42693c83a563 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:20:38Z |
| publishDate | 2003 |
| record_format | dspace |
| spelling | oxford-uuid:b74a8e9a-8daa-4ae8-8b9a-42693c83a5632022-03-27T04:47:30ZEvaluation of ovotransferrin-matrix metal-affinity metalloprotein chromatography for Pu(IV) separationsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:b74a8e9a-8daa-4ae8-8b9a-42693c83a563EnglishSymplectic Elements at Oxford2003Myers, WRuggiero, CReilly, SNeu, MMetal-Affinity metalloprotein chromatography has been suggested to have potential for selectively removing actinides from solution. To evaluate this potential, characterization of Pu(IV) and Fe(III) binding to an ovotransferrin-Sepharos™ 4B matrix, with oxalate and carbonate synergistic anions, was performed. Metal binding capacity was determined for metal concentrations ranging from 2 × 10-6 to 1 × 10 -3 M Fe(III) and 2.9 × 10-5 to 2.6 × 10 -4 M Pu(IV), where both metals were added as a solution of 10:1 nitrilotriacetic acid:metal. Metal binding capacity was also determined over the pH range 3.0 to 9.2. The metal binding properties of the immobilized ovotransferrin were found to differ from those reported for free-protein studies. Distribution coefficients were low: for metal solution concentrations of 0.02, 0.04, 0.10, and 0.20 mM, coefficients were 540, 180, 23, and 12mL/g for Fe(III) and 20, 12, 7.2, and 6.0 mL/g for Pu(IV), respectively. The capacity of the material was less dependent on pH than expected, varying by only two μmoles metal/g matrix for both Fe(III) and Pu(IV) over the pH range of 4.2 to 9.5. This study illustrates limitations of metalloprotein chromatography for Pu(IV) separation. |
| spellingShingle | Myers, W Ruggiero, C Reilly, S Neu, M Evaluation of ovotransferrin-matrix metal-affinity metalloprotein chromatography for Pu(IV) separations |
| title | Evaluation of ovotransferrin-matrix metal-affinity metalloprotein chromatography for Pu(IV) separations |
| title_full | Evaluation of ovotransferrin-matrix metal-affinity metalloprotein chromatography for Pu(IV) separations |
| title_fullStr | Evaluation of ovotransferrin-matrix metal-affinity metalloprotein chromatography for Pu(IV) separations |
| title_full_unstemmed | Evaluation of ovotransferrin-matrix metal-affinity metalloprotein chromatography for Pu(IV) separations |
| title_short | Evaluation of ovotransferrin-matrix metal-affinity metalloprotein chromatography for Pu(IV) separations |
| title_sort | evaluation of ovotransferrin matrix metal affinity metalloprotein chromatography for pu iv separations |
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