Coordinated control XerC and XerD catalytic during holliday junction resolution
Site-specific recombinases XerC and XerD function in the segregation of circular bacterial replicons. In a recombining nucleoprotein complex containing two molecules each of XerC and XerD, coordinated reciprocal switches in recombinase activity ensure that only XerC or XerD is active at any one time...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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2000
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| _version_ | 1826292921343672320 |
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| author | Arciszewska, L Baker, R Hallet, B Sherratt, D |
| author_facet | Arciszewska, L Baker, R Hallet, B Sherratt, D |
| author_sort | Arciszewska, L |
| collection | OXFORD |
| description | Site-specific recombinases XerC and XerD function in the segregation of circular bacterial replicons. In a recombining nucleoprotein complex containing two molecules each of XerC and XerD, coordinated reciprocal switches in recombinase activity ensure that only XerC or XerD is active at any one time. Mutated recombinases that carry substitutions of a catalytic arginine residue stimulate cleavage and strand exchange mediated by the partner recombinase on DNA substrates that are normally recombined poorly by the partner. This is associated with a reciprocal impairment of the recombinase's own ability to initiate catalysis. The extent of this switch in catalysis is modulated by changes in recombination site sequence and is not a direct consequence of any catalytic defect. We propose that altered interactions between the mutated proteins and their wild-type partners lead to an increased level of an alternative Holliday junction intermediate that has a conformation appropriate for resolution by the partner recombinase. The results indicate how subtle changes in protein-DNA architecture at a Holliday junction can redirect recombination outcome. (C) 2000 Academic Press. |
| first_indexed | 2024-03-07T03:22:08Z |
| format | Journal article |
| id | oxford-uuid:b7caa5bf-56ca-4f6b-886b-86ad3d8d35a7 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:22:08Z |
| publishDate | 2000 |
| record_format | dspace |
| spelling | oxford-uuid:b7caa5bf-56ca-4f6b-886b-86ad3d8d35a72022-03-27T04:51:10ZCoordinated control XerC and XerD catalytic during holliday junction resolutionJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:b7caa5bf-56ca-4f6b-886b-86ad3d8d35a7EnglishSymplectic Elements at Oxford2000Arciszewska, LBaker, RHallet, BSherratt, DSite-specific recombinases XerC and XerD function in the segregation of circular bacterial replicons. In a recombining nucleoprotein complex containing two molecules each of XerC and XerD, coordinated reciprocal switches in recombinase activity ensure that only XerC or XerD is active at any one time. Mutated recombinases that carry substitutions of a catalytic arginine residue stimulate cleavage and strand exchange mediated by the partner recombinase on DNA substrates that are normally recombined poorly by the partner. This is associated with a reciprocal impairment of the recombinase's own ability to initiate catalysis. The extent of this switch in catalysis is modulated by changes in recombination site sequence and is not a direct consequence of any catalytic defect. We propose that altered interactions between the mutated proteins and their wild-type partners lead to an increased level of an alternative Holliday junction intermediate that has a conformation appropriate for resolution by the partner recombinase. The results indicate how subtle changes in protein-DNA architecture at a Holliday junction can redirect recombination outcome. (C) 2000 Academic Press. |
| spellingShingle | Arciszewska, L Baker, R Hallet, B Sherratt, D Coordinated control XerC and XerD catalytic during holliday junction resolution |
| title | Coordinated control XerC and XerD catalytic during holliday junction resolution |
| title_full | Coordinated control XerC and XerD catalytic during holliday junction resolution |
| title_fullStr | Coordinated control XerC and XerD catalytic during holliday junction resolution |
| title_full_unstemmed | Coordinated control XerC and XerD catalytic during holliday junction resolution |
| title_short | Coordinated control XerC and XerD catalytic during holliday junction resolution |
| title_sort | coordinated control xerc and xerd catalytic during holliday junction resolution |
| work_keys_str_mv | AT arciszewskal coordinatedcontrolxercandxerdcatalyticduringhollidayjunctionresolution AT bakerr coordinatedcontrolxercandxerdcatalyticduringhollidayjunctionresolution AT halletb coordinatedcontrolxercandxerdcatalyticduringhollidayjunctionresolution AT sherrattd coordinatedcontrolxercandxerdcatalyticduringhollidayjunctionresolution |