Nanopore sequencing of single-cell transcriptomes with scCOLOR-seq

Here we describe single-cell corrected long-read sequencing (scCOLOR-seq), which enables error correction of barcode and unique molecular identifier oligonucleotide sequences and permits standalone cDNA nanopore sequencing of single cells. Barcodes and unique molecular identifiers are synthesized us...

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Detalhes bibliográficos
Principais autores: Philpott, M, Watson, J, Thakurta, A, Brown, T, Oppermann, U, Cribbs, AP
Formato: Journal article
Idioma:English
Publicado em: Springer Nature 2021