Selective photostimulation of genetically chARGed neurons.
To permit direct functional analyses of neural circuits, we have developed a method for stimulating groups of genetically designated neurons optically. Coexpression of the Drosophila photoreceptor genes encoding arrestin-2, rhodopsin (formed by liganding opsin with retinal), and the alpha subunit of...
| المؤلفون الرئيسيون: | , , , |
|---|---|
| التنسيق: | Journal article |
| اللغة: | English |
| منشور في: |
2002
|
| _version_ | 1826293025628749824 |
|---|---|
| author | Zemelman, B Lee, G Ng, M Miesenböck, G |
| author_facet | Zemelman, B Lee, G Ng, M Miesenböck, G |
| author_sort | Zemelman, B |
| collection | OXFORD |
| description | To permit direct functional analyses of neural circuits, we have developed a method for stimulating groups of genetically designated neurons optically. Coexpression of the Drosophila photoreceptor genes encoding arrestin-2, rhodopsin (formed by liganding opsin with retinal), and the alpha subunit of the cognate heterotrimeric G protein--an explosive combination we term "chARGe"--sensitizes generalist vertebrate neurons to light. Illumination of a mixed population of neurons elicits action potentials selectively and cell-autonomously in its genetically chARGed members. In contrast to bath-applied photostimulants or caged neurotransmitters, which act indiscriminately throughout the illuminated volume, chARGe localizes the responsiveness to light. Distributed activity may thus be fed directly into a circumscribed population of neurons in intact tissue, irrespective of the spatial arrangement of its elements. |
| first_indexed | 2024-03-07T03:23:44Z |
| format | Journal article |
| id | oxford-uuid:b852d875-9ac3-4722-8ab8-0bca049e9974 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:23:44Z |
| publishDate | 2002 |
| record_format | dspace |
| spelling | oxford-uuid:b852d875-9ac3-4722-8ab8-0bca049e99742022-03-27T04:55:01ZSelective photostimulation of genetically chARGed neurons.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:b852d875-9ac3-4722-8ab8-0bca049e9974EnglishSymplectic Elements at Oxford2002Zemelman, BLee, GNg, MMiesenböck, GTo permit direct functional analyses of neural circuits, we have developed a method for stimulating groups of genetically designated neurons optically. Coexpression of the Drosophila photoreceptor genes encoding arrestin-2, rhodopsin (formed by liganding opsin with retinal), and the alpha subunit of the cognate heterotrimeric G protein--an explosive combination we term "chARGe"--sensitizes generalist vertebrate neurons to light. Illumination of a mixed population of neurons elicits action potentials selectively and cell-autonomously in its genetically chARGed members. In contrast to bath-applied photostimulants or caged neurotransmitters, which act indiscriminately throughout the illuminated volume, chARGe localizes the responsiveness to light. Distributed activity may thus be fed directly into a circumscribed population of neurons in intact tissue, irrespective of the spatial arrangement of its elements. |
| spellingShingle | Zemelman, B Lee, G Ng, M Miesenböck, G Selective photostimulation of genetically chARGed neurons. |
| title | Selective photostimulation of genetically chARGed neurons. |
| title_full | Selective photostimulation of genetically chARGed neurons. |
| title_fullStr | Selective photostimulation of genetically chARGed neurons. |
| title_full_unstemmed | Selective photostimulation of genetically chARGed neurons. |
| title_short | Selective photostimulation of genetically chARGed neurons. |
| title_sort | selective photostimulation of genetically charged neurons |
| work_keys_str_mv | AT zemelmanb selectivephotostimulationofgeneticallychargedneurons AT leeg selectivephotostimulationofgeneticallychargedneurons AT ngm selectivephotostimulationofgeneticallychargedneurons AT miesenbockg selectivephotostimulationofgeneticallychargedneurons |