A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1.
Homotypic adhesion by phorbol ester-stimulated lymphocytes requires LFA-1 and Mg+2 and does not involve like-like interactions between LFA-1 molecules on adjacent cells. The latter finding suggested that a second molecule, distinct from LFA-1, also participates in LFA-1-dependent adhesion. The ident...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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1986
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| _version_ | 1826293029365874688 |
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| author | Rothlein, R Dustin, M Marlin, S Springer, T |
| author_facet | Rothlein, R Dustin, M Marlin, S Springer, T |
| author_sort | Rothlein, R |
| collection | OXFORD |
| description | Homotypic adhesion by phorbol ester-stimulated lymphocytes requires LFA-1 and Mg+2 and does not involve like-like interactions between LFA-1 molecules on adjacent cells. The latter finding suggested that a second molecule, distinct from LFA-1, also participates in LFA-1-dependent adhesion. The identification of such a molecule was the object of this investigation. After immunization with LFA-1-deficient EBV-transformed lymphoblastoid cells, a MAb was obtained that inhibits phorbol ester-stimulated aggregation of LFA-1+ EBV lines. This MAb defines a novel cell surface molecule, which is designated intercellular adhesion molecule 1 (ICAM-1). ICAM-1 is distinct from LFA-1 in both cell distribution and structure. In SDS-PAGE, ICAM-1 isolated from JY cells is a single chain of Mr = 90,000. As shown by MAb inhibition, ICAM-1 participates in phorbol ester-stimulated adhesion reactions of B lymphocyte and myeloid cell lines and T lymphocyte blasts. However, aggregation of one T lymphocyte cell line (SKW-3) was inhibited by LFA-1 but not ICAM-1 MAb. It is proposed that ICAM-1 may be a ligand in many, but not all, LFA-1-dependent adhesion reactions. |
| first_indexed | 2024-03-07T03:23:47Z |
| format | Journal article |
| id | oxford-uuid:b856ddef-1b1b-4d55-9983-c3893431e527 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:23:47Z |
| publishDate | 1986 |
| record_format | dspace |
| spelling | oxford-uuid:b856ddef-1b1b-4d55-9983-c3893431e5272022-03-27T04:55:08ZA human intercellular adhesion molecule (ICAM-1) distinct from LFA-1.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:b856ddef-1b1b-4d55-9983-c3893431e527EnglishSymplectic Elements at Oxford1986Rothlein, RDustin, MMarlin, SSpringer, THomotypic adhesion by phorbol ester-stimulated lymphocytes requires LFA-1 and Mg+2 and does not involve like-like interactions between LFA-1 molecules on adjacent cells. The latter finding suggested that a second molecule, distinct from LFA-1, also participates in LFA-1-dependent adhesion. The identification of such a molecule was the object of this investigation. After immunization with LFA-1-deficient EBV-transformed lymphoblastoid cells, a MAb was obtained that inhibits phorbol ester-stimulated aggregation of LFA-1+ EBV lines. This MAb defines a novel cell surface molecule, which is designated intercellular adhesion molecule 1 (ICAM-1). ICAM-1 is distinct from LFA-1 in both cell distribution and structure. In SDS-PAGE, ICAM-1 isolated from JY cells is a single chain of Mr = 90,000. As shown by MAb inhibition, ICAM-1 participates in phorbol ester-stimulated adhesion reactions of B lymphocyte and myeloid cell lines and T lymphocyte blasts. However, aggregation of one T lymphocyte cell line (SKW-3) was inhibited by LFA-1 but not ICAM-1 MAb. It is proposed that ICAM-1 may be a ligand in many, but not all, LFA-1-dependent adhesion reactions. |
| spellingShingle | Rothlein, R Dustin, M Marlin, S Springer, T A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1. |
| title | A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1. |
| title_full | A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1. |
| title_fullStr | A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1. |
| title_full_unstemmed | A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1. |
| title_short | A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1. |
| title_sort | human intercellular adhesion molecule icam 1 distinct from lfa 1 |
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