Extraction of a synthetic androgen from fish muscle and quantitation by high performance liquid chromatography.
A rapid, reproducible extraction procedure was developed to recover the synthetic androgen 17 alpha-methyltestosterone (MT) from fish muscle. A single extraction with chloroform-methanol (2:1 V/V), followed by elution from mini-columns with various methanol concentrations, yielded an extraction effi...
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| Format: | Journal article |
| Language: | English |
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1984
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| _version_ | 1797090928344694784 |
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| author | Goudie, C |
| author_facet | Goudie, C |
| author_sort | Goudie, C |
| collection | OXFORD |
| description | A rapid, reproducible extraction procedure was developed to recover the synthetic androgen 17 alpha-methyltestosterone (MT) from fish muscle. A single extraction with chloroform-methanol (2:1 V/V), followed by elution from mini-columns with various methanol concentrations, yielded an extraction efficiency of greater than 70%. Resolution of MT from testosterone was complete and reliable quantitation was achieved utilizing reversed-phase high performance liquid chromatography. Endogenous testosterone was not detectable in 1-g muscle samples, but MT was detected in fish muscle 6 h after adult Tilapia aurea were fed a diet containing the steroid. |
| first_indexed | 2024-03-07T03:25:41Z |
| format | Journal article |
| id | oxford-uuid:b8f359ba-f772-4f1e-9860-41fcc056570b |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:25:41Z |
| publishDate | 1984 |
| record_format | dspace |
| spelling | oxford-uuid:b8f359ba-f772-4f1e-9860-41fcc056570b2022-03-27T04:59:28ZExtraction of a synthetic androgen from fish muscle and quantitation by high performance liquid chromatography.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:b8f359ba-f772-4f1e-9860-41fcc056570bEnglishSymplectic Elements at Oxford1984Goudie, CA rapid, reproducible extraction procedure was developed to recover the synthetic androgen 17 alpha-methyltestosterone (MT) from fish muscle. A single extraction with chloroform-methanol (2:1 V/V), followed by elution from mini-columns with various methanol concentrations, yielded an extraction efficiency of greater than 70%. Resolution of MT from testosterone was complete and reliable quantitation was achieved utilizing reversed-phase high performance liquid chromatography. Endogenous testosterone was not detectable in 1-g muscle samples, but MT was detected in fish muscle 6 h after adult Tilapia aurea were fed a diet containing the steroid. |
| spellingShingle | Goudie, C Extraction of a synthetic androgen from fish muscle and quantitation by high performance liquid chromatography. |
| title | Extraction of a synthetic androgen from fish muscle and quantitation by high performance liquid chromatography. |
| title_full | Extraction of a synthetic androgen from fish muscle and quantitation by high performance liquid chromatography. |
| title_fullStr | Extraction of a synthetic androgen from fish muscle and quantitation by high performance liquid chromatography. |
| title_full_unstemmed | Extraction of a synthetic androgen from fish muscle and quantitation by high performance liquid chromatography. |
| title_short | Extraction of a synthetic androgen from fish muscle and quantitation by high performance liquid chromatography. |
| title_sort | extraction of a synthetic androgen from fish muscle and quantitation by high performance liquid chromatography |
| work_keys_str_mv | AT goudiec extractionofasyntheticandrogenfromfishmuscleandquantitationbyhighperformanceliquidchromatography |