Immune signaling pathways regulating bacterial and malaria parasite infection of the mosquito Anopheles gambiae.
We show that, in the malaria vector Anopheles gambiae, expression of Cecropin 1 is regulated by REL2, an NF-kappaB-like transcription factor orthologous to Drosophila Relish. Through alternative splicing, REL2 produces a full-length (REL2-F) and a shorter (REL2-S) protein isoform lacking the inhibit...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2005
|
| _version_ | 1826293167533588480 |
|---|---|
| author | Meister, S Kanzok, S Zheng, X Luna, C Li, T Hoa, N Clayton, JR White, K Kafatos, F Christophides, G Zheng, L |
| author_facet | Meister, S Kanzok, S Zheng, X Luna, C Li, T Hoa, N Clayton, JR White, K Kafatos, F Christophides, G Zheng, L |
| author_sort | Meister, S |
| collection | OXFORD |
| description | We show that, in the malaria vector Anopheles gambiae, expression of Cecropin 1 is regulated by REL2, an NF-kappaB-like transcription factor orthologous to Drosophila Relish. Through alternative splicing, REL2 produces a full-length (REL2-F) and a shorter (REL2-S) protein isoform lacking the inhibitory ankyrin repeats and death domain. RNA interference experiments show that, in contrast to Drosophila Relish, which responds solely to Gram-negative bacteria, the Anopheles REL2-F and REL2-S isoforms are involved in defense against the Gram-positive Staphylococcus aureus and the Gram-negative Escherichia coli bacteria, respectively. REL2-F also regulates the intensity of mosquito infection with the malaria parasite, Plasmodium berghei. The adaptor IMD shares the same activities as REL2-F. Microarray analysis identified 10 additional genes regulated by REL2, including CEC3, GAM1, and LRIM1. |
| first_indexed | 2024-03-07T03:25:56Z |
| format | Journal article |
| id | oxford-uuid:b9079265-83ac-4786-867e-907dbd7ed723 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:25:56Z |
| publishDate | 2005 |
| record_format | dspace |
| spelling | oxford-uuid:b9079265-83ac-4786-867e-907dbd7ed7232022-03-27T05:00:08ZImmune signaling pathways regulating bacterial and malaria parasite infection of the mosquito Anopheles gambiae.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:b9079265-83ac-4786-867e-907dbd7ed723EnglishSymplectic Elements at Oxford2005Meister, SKanzok, SZheng, XLuna, CLi, THoa, NClayton, JRWhite, KKafatos, FChristophides, GZheng, LWe show that, in the malaria vector Anopheles gambiae, expression of Cecropin 1 is regulated by REL2, an NF-kappaB-like transcription factor orthologous to Drosophila Relish. Through alternative splicing, REL2 produces a full-length (REL2-F) and a shorter (REL2-S) protein isoform lacking the inhibitory ankyrin repeats and death domain. RNA interference experiments show that, in contrast to Drosophila Relish, which responds solely to Gram-negative bacteria, the Anopheles REL2-F and REL2-S isoforms are involved in defense against the Gram-positive Staphylococcus aureus and the Gram-negative Escherichia coli bacteria, respectively. REL2-F also regulates the intensity of mosquito infection with the malaria parasite, Plasmodium berghei. The adaptor IMD shares the same activities as REL2-F. Microarray analysis identified 10 additional genes regulated by REL2, including CEC3, GAM1, and LRIM1. |
| spellingShingle | Meister, S Kanzok, S Zheng, X Luna, C Li, T Hoa, N Clayton, JR White, K Kafatos, F Christophides, G Zheng, L Immune signaling pathways regulating bacterial and malaria parasite infection of the mosquito Anopheles gambiae. |
| title | Immune signaling pathways regulating bacterial and malaria parasite infection of the mosquito Anopheles gambiae. |
| title_full | Immune signaling pathways regulating bacterial and malaria parasite infection of the mosquito Anopheles gambiae. |
| title_fullStr | Immune signaling pathways regulating bacterial and malaria parasite infection of the mosquito Anopheles gambiae. |
| title_full_unstemmed | Immune signaling pathways regulating bacterial and malaria parasite infection of the mosquito Anopheles gambiae. |
| title_short | Immune signaling pathways regulating bacterial and malaria parasite infection of the mosquito Anopheles gambiae. |
| title_sort | immune signaling pathways regulating bacterial and malaria parasite infection of the mosquito anopheles gambiae |
| work_keys_str_mv | AT meisters immunesignalingpathwaysregulatingbacterialandmalariaparasiteinfectionofthemosquitoanophelesgambiae AT kanzoks immunesignalingpathwaysregulatingbacterialandmalariaparasiteinfectionofthemosquitoanophelesgambiae AT zhengx immunesignalingpathwaysregulatingbacterialandmalariaparasiteinfectionofthemosquitoanophelesgambiae AT lunac immunesignalingpathwaysregulatingbacterialandmalariaparasiteinfectionofthemosquitoanophelesgambiae AT lit immunesignalingpathwaysregulatingbacterialandmalariaparasiteinfectionofthemosquitoanophelesgambiae AT hoan immunesignalingpathwaysregulatingbacterialandmalariaparasiteinfectionofthemosquitoanophelesgambiae AT claytonjr immunesignalingpathwaysregulatingbacterialandmalariaparasiteinfectionofthemosquitoanophelesgambiae AT whitek immunesignalingpathwaysregulatingbacterialandmalariaparasiteinfectionofthemosquitoanophelesgambiae AT kafatosf immunesignalingpathwaysregulatingbacterialandmalariaparasiteinfectionofthemosquitoanophelesgambiae AT christophidesg immunesignalingpathwaysregulatingbacterialandmalariaparasiteinfectionofthemosquitoanophelesgambiae AT zhengl immunesignalingpathwaysregulatingbacterialandmalariaparasiteinfectionofthemosquitoanophelesgambiae |