Isolation of probes specific to human chromosomal region 6p21 from immunoselected irradiation-fusion gene transfer hybrids.
A hybrid cell line (R21/B1) containing a truncated human chromosome 6 (6pter-6q21) and a human Y chromosome on a hamster background was irradiated and fused to A23 (TK-) or W3GH (HPRT-) hamster cells. Clones containing expressed HLA class I genes (4/40) were selected using monoclonal antibodies. The...
Main Authors: | Ragoussis, I, Jones, T, Sheer, D, Shrimpton, A, Goodfellow, P, Trowsdale, J, Ziegler, A |
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Format: | Journal article |
Language: | English |
Published: |
1991
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