GPRC5B a putative glutamate-receptor candidate is negative modulator of insulin secretion.
GPRC5B is an orphan receptor belonging to the group C family of G protein-coupled receptors (GPCRs). GPRC5B is abundantly expressed in both human and mouse pancreatic islets, and both GPRC5B mRNA and protein are up-regulated 2.5-fold in islets from organ donors with type 2 diabetes. Expression of Gp...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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2013
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| _version_ | 1797106520386699264 |
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| author | Soni, A Amisten, S Rorsman, P Salehi, A |
| author_facet | Soni, A Amisten, S Rorsman, P Salehi, A |
| author_sort | Soni, A |
| collection | OXFORD |
| description | GPRC5B is an orphan receptor belonging to the group C family of G protein-coupled receptors (GPCRs). GPRC5B is abundantly expressed in both human and mouse pancreatic islets, and both GPRC5B mRNA and protein are up-regulated 2.5-fold in islets from organ donors with type 2 diabetes. Expression of Gprc5b is 50% lower in islets isolated from newborn (<3 weeks) than in adult (>36 weeks) mice. Lentiviral shRNA-mediated down-regulation of Gprc5b in intact islets from 12-16 week old mice strongly (2.5-fold) increased basal (1 mmol/l) and moderately (40%) potentiated glucose-(20 mmol/l) stimulated insulin secretion and also enhanced the potentiating effect of glutamate on insulin secretion. Down-regulation of Gprc5b protected murine insulin-secreting clonal MIN6 cells against cytokine-induced apoptosis. We propose that increased expression of GPRC5B contributes to the reduced insulin secretion and β-cell viability observed in type-2 diabetes. Thus, pharmacological targeting of GPRC5B might provide a novel means therapy for the treatment and prevention of type 2 diabetes. |
| first_indexed | 2024-03-07T07:01:38Z |
| format | Journal article |
| id | oxford-uuid:b9c53542-5bb7-4913-bee1-c93210ed461c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:01:38Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | oxford-uuid:b9c53542-5bb7-4913-bee1-c93210ed461c2022-03-29T17:18:48ZGPRC5B a putative glutamate-receptor candidate is negative modulator of insulin secretion.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:b9c53542-5bb7-4913-bee1-c93210ed461cEnglishSymplectic Elements at Oxford2013Soni, AAmisten, SRorsman, PSalehi, AGPRC5B is an orphan receptor belonging to the group C family of G protein-coupled receptors (GPCRs). GPRC5B is abundantly expressed in both human and mouse pancreatic islets, and both GPRC5B mRNA and protein are up-regulated 2.5-fold in islets from organ donors with type 2 diabetes. Expression of Gprc5b is 50% lower in islets isolated from newborn (<3 weeks) than in adult (>36 weeks) mice. Lentiviral shRNA-mediated down-regulation of Gprc5b in intact islets from 12-16 week old mice strongly (2.5-fold) increased basal (1 mmol/l) and moderately (40%) potentiated glucose-(20 mmol/l) stimulated insulin secretion and also enhanced the potentiating effect of glutamate on insulin secretion. Down-regulation of Gprc5b protected murine insulin-secreting clonal MIN6 cells against cytokine-induced apoptosis. We propose that increased expression of GPRC5B contributes to the reduced insulin secretion and β-cell viability observed in type-2 diabetes. Thus, pharmacological targeting of GPRC5B might provide a novel means therapy for the treatment and prevention of type 2 diabetes. |
| spellingShingle | Soni, A Amisten, S Rorsman, P Salehi, A GPRC5B a putative glutamate-receptor candidate is negative modulator of insulin secretion. |
| title | GPRC5B a putative glutamate-receptor candidate is negative modulator of insulin secretion. |
| title_full | GPRC5B a putative glutamate-receptor candidate is negative modulator of insulin secretion. |
| title_fullStr | GPRC5B a putative glutamate-receptor candidate is negative modulator of insulin secretion. |
| title_full_unstemmed | GPRC5B a putative glutamate-receptor candidate is negative modulator of insulin secretion. |
| title_short | GPRC5B a putative glutamate-receptor candidate is negative modulator of insulin secretion. |
| title_sort | gprc5b a putative glutamate receptor candidate is negative modulator of insulin secretion |
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