Low-speed centrifugation of retroviral vectors absorbed to a particulate substrate: a highly effective means of enhancing retroviral titre.

Low-speed centrifugation of retroviral vectors absorbed to a particulate substrate: a highly effective means of enhancing retroviral titre.

For many gene therapy applications the effective titre of retroviral vectors is a limiting factor both in vitro and in vivo. Purification and concentration of retrovirus from packaging cell supernatant can overcome this problem. To this end we have investigated a novel procedure which involves compl...

पूर्ण विवरण

ग्रंथसूची विवरण
मुख्य लेखकों:	Darling, D, Hughes, C, Galea-Lauri, J, Gäken, J, Trayner, I, Kuiper, M, Farzaneh, F
स्वरूप:	Journal article
भाषा:	English
प्रकाशित:	2000

समान संसाधन

A polycistronic retroviral vector encoding hB7-1 (CD80) and hIL-2 for ex vivo cancer gene therapy.
द्वारा: Kuiper, M, और अन्य
प्रकाशित: (1996)

Affinity mediated magnetic concentration of retroviral vectors
द्वारा: Hughes, C, और अन्य
प्रकाशित: (2001)

Streptavidin paramagnetic particles provide a choice of three affinity-based capture and magnetic concentration strategies for retroviral vectors.
द्वारा: Hughes, C, और अन्य
प्रकाशित: (2001)

High titre vectors for targeted expression of multiple genes
द्वारा: Darling, D, और अन्य
प्रकाशित: (2004)

Monocistronic high titre vectors for targeted expression of multiple therapeutic genes
द्वारा: Darling, D, और अन्य
प्रकाशित: (2004)

Efficient clinical-grade γ-retroviral vector purification by high-speed centrifugation for CAR T cell manufacturing
द्वारा: Leila Mekkaoui, और अन्य
प्रकाशित: (2023-03-01)

Efficient retroviral infection of mammalian cells is blocked by inhibition of poly(ADP-ribose) polymerase activity.
द्वारा: Gäken, J, और अन्य
प्रकाशित: (1996)

Cloning and Characterization of a Retroviral Plasmid, pCC1, for Combination Suicide Gene Therapy
द्वारा: Marcel Kuiper, और अन्य
प्रकाशित: (2000-03-01)

Retroviral vectors for use in human gene therapy for cancer, Gaucher disease, and arthritis.
द्वारा: Robbins, P, और अन्य
प्रकाशित: (1994)

Restriction Mapping of Retroviral Vector Episomal DNA
द्वारा: Won-Bin Young, और अन्य
प्रकाशित: (2000-03-01)

Comparative Studies on Retroviral Proteases: Substrate Specificity
द्वारा: József Tözsér
प्रकाशित: (2010-01-01)

Q Vectors, Bicistronic Retroviral Vectors for Gene Transfer
द्वारा: Martin A. Julius, और अन्य
प्रकाशित: (2000-04-01)

Gene expression studies using retroviral vectors
द्वारा: Cone, Roger David.
प्रकाशित: (2024)

Retroviral and Lentiviral Vectors for the Induction of Immunological Tolerance
द्वारा: Inès Dufait, और अन्य
प्रकाशित: (2012-01-01)

Retroviral Vectors for Analysis of Viral Mutagenesis and Recombination
द्वारा: Jonathan M.O. Rawson, और अन्य
प्रकाशित: (2014-09-01)

Enhanced gene expression from retroviral vectors
द्वारा: Micklem David R, और अन्य
प्रकाशित: (2008-02-01)

Retroviral Transfection
द्वारा: Kristie Nybo
प्रकाशित: (2012-08-01)

Retroviral Gene Vectors Show Clear Target Preferences.
प्रकाशित: (2004-12-01)

Retroviral Vectors: Post Entry Events and Genomic Alterations
द्वारा: Christof von Kalle, और अन्य
प्रकाशित: (2011-04-01)

Applications of RNA structure analysis to retroviral packaging and anti-retroviral therapeutic discovery
द्वारा: Rein Alan, और अन्य
प्रकाशित: (2011-10-01)

Retroviral RNA Processing
द्वारा: Karen L. Beemon
प्रकाशित: (2022-05-01)

Retroviral superinfection resistance
द्वारा: van der Kuyl Antoinette C, और अन्य
प्रकाशित: (2005-08-01)

Regulatory T cells in retroviral infections.
द्वारा: Kim J Hasenkrug, और अन्य
प्रकाशित: (2018-02-01)

Insertional mutagenesis in zebrafish using a pseudotyped retroviral vector
द्वारा: Gaiano, Nicholas R. (Nicholas Roger)
प्रकाशित: (2008)

An XMRV Derived Retroviral Vector as a Tool for Gene Transfer
द्वारा: Rojas-Martinez Augusto, और अन्य
प्रकाशित: (2011-06-01)

A new generation of pPRIG-based retroviral vectors
द्वारा: Boulukos Kim E, और अन्य
प्रकाशित: (2007-11-01)

Analyzing the Genotoxicity of Retroviral Vectors in Hematopoietic Cell Gene Therapy
द्वारा: Luca Biasco, और अन्य
प्रकाशित: (2018-03-01)

Use of Endogenous Retroviral Sequences (ERVs) and structural markers for retroviral phylogenetic inference and taxonomy
द्वारा: Sperber Göran O, और अन्य
प्रकाशित: (2005-08-01)

Tumour cells expressing B7.1 costimulatory molecule: Are these better targets for NK cells?
द्वारा: GaleaLauri, J, और अन्य
प्रकाशित: (1996)

Therapeutic aspects of retroviral disease.
द्वारा: Peto, T
प्रकाशित: (1992)

Silencing of Unintegrated Retroviral DNAs
द्वारा: Stephen P. Goff
प्रकाशित: (2021-11-01)

Are dogs not susceptible to retroviral infections?
द्वारा: Jorge Casseb, और अन्य
प्रकाशित: (2023-10-01)

Stephen Oroszlan and Retroviral Proteins
द्वारा: Alan Rein
प्रकाशित: (2022-01-01)

The Host RNAs in Retroviral Particles
द्वारा: Alice Telesnitsky, और अन्य
प्रकाशित: (2016-08-01)

Pathogenetic aspects of retroviral infections
द्वारा: L. P. Horalskyi, और अन्य
प्रकाशित: (2022-04-01)

Special Issue: Retroviral Enzymes
द्वारा: Luis Menéndez-Arias
प्रकाशित: (2010-05-01)

Retroviral Integration Site Selection
द्वारा: Angela Ciuffi, और अन्य
प्रकाशित: (2010-01-01)

An Enhanced Retroviral Vector for Efficient Genetic Manipulation and Selection in Mammalian Cells
द्वारा: Jana Triller, और अन्य
प्रकाशित: (2024-09-01)

Functional Testing of a Bicistronic Retroviral Vector for Intracellular Peptide Production
द्वारा: M. Duch, और अन्य
प्रकाशित: (1999-06-01)

Endogenous non-retroviral elements in genomes of Aedes mosquitoes and vector competence
द्वारा: Vincent Houé, और अन्य
प्रकाशित: (2019-01-01)