Factors governing attachment of Rhizobium leguminosarum to legume roots at acid, neutral, and alkaline pHs
Rhizobial attachment to host legume roots is the first physical interaction of bacteria and plants in symbiotic nitrogen fixation. The pH-dependent primary attachment of <em>Rhizobium leguminosarum</em> biovar viciae 3841 to <em>Pisum sativum</em> (pea) roots was investigated...
| Päätekijät: | , , , , , , , |
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| Aineistotyyppi: | Journal article |
| Kieli: | English |
| Julkaistu: |
American Society for Microbiology
2024
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| _version_ | 1826317648557768704 |
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| author | Parsons, JD Cocker, CR East, AK Wheatley, RM Ramachandran, VK Kaschani, F Kaiser, M Poole, PS |
| author_facet | Parsons, JD Cocker, CR East, AK Wheatley, RM Ramachandran, VK Kaschani, F Kaiser, M Poole, PS |
| author_sort | Parsons, JD |
| collection | OXFORD |
| description | Rhizobial attachment to host legume roots is the first physical interaction of bacteria and plants in symbiotic nitrogen fixation. The pH-dependent primary attachment of <em>Rhizobium leguminosarum</em> biovar viciae 3841 to <em>Pisum sativum</em> (pea) roots was investigated by genome-wide insertion sequencing, luminescence-based attachment assays, and proteomic analysis. Under acid, neutral, or alkaline pH, a total of 115 genes are needed for primary attachment under one or more environmental pH, with 22 genes required for all. These include components of cell surfaces and membranes, together with enzymes that construct and modify them. Mechanisms of dealing with stress also play a part; however, exact requirements vary depending on environmental pH. RNASeq showed that knocking out the two transcriptional regulators required for attachment causes massive changes in the bacterial cell surface. Approximately half of the 54 proteins required for attachment at pH 7.0 have a role in the later stages of nodule formation. We found no evidence for a single rhicadhesin responsible for alkaline attachment, although sonicated cell surface fractions inhibited root attachment at alkaline pH. Our results demonstrate the complexity of primary root attachment and illustrate the diversity of mechanisms involved. |
| first_indexed | 2025-03-11T16:57:15Z |
| format | Journal article |
| id | oxford-uuid:ba3f0b8c-a00d-49c4-9c75-d29ddfe5b0f5 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2025-03-11T16:57:15Z |
| publishDate | 2024 |
| publisher | American Society for Microbiology |
| record_format | dspace |
| spelling | oxford-uuid:ba3f0b8c-a00d-49c4-9c75-d29ddfe5b0f52025-02-25T12:32:53ZFactors governing attachment of Rhizobium leguminosarum to legume roots at acid, neutral, and alkaline pHsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ba3f0b8c-a00d-49c4-9c75-d29ddfe5b0f5EnglishSymplectic ElementsAmerican Society for Microbiology2024Parsons, JDCocker, CREast, AKWheatley, RMRamachandran, VKKaschani, FKaiser, MPoole, PSRhizobial attachment to host legume roots is the first physical interaction of bacteria and plants in symbiotic nitrogen fixation. The pH-dependent primary attachment of <em>Rhizobium leguminosarum</em> biovar viciae 3841 to <em>Pisum sativum</em> (pea) roots was investigated by genome-wide insertion sequencing, luminescence-based attachment assays, and proteomic analysis. Under acid, neutral, or alkaline pH, a total of 115 genes are needed for primary attachment under one or more environmental pH, with 22 genes required for all. These include components of cell surfaces and membranes, together with enzymes that construct and modify them. Mechanisms of dealing with stress also play a part; however, exact requirements vary depending on environmental pH. RNASeq showed that knocking out the two transcriptional regulators required for attachment causes massive changes in the bacterial cell surface. Approximately half of the 54 proteins required for attachment at pH 7.0 have a role in the later stages of nodule formation. We found no evidence for a single rhicadhesin responsible for alkaline attachment, although sonicated cell surface fractions inhibited root attachment at alkaline pH. Our results demonstrate the complexity of primary root attachment and illustrate the diversity of mechanisms involved. |
| spellingShingle | Parsons, JD Cocker, CR East, AK Wheatley, RM Ramachandran, VK Kaschani, F Kaiser, M Poole, PS Factors governing attachment of Rhizobium leguminosarum to legume roots at acid, neutral, and alkaline pHs |
| title | Factors governing attachment of Rhizobium leguminosarum to legume roots at acid, neutral, and alkaline pHs |
| title_full | Factors governing attachment of Rhizobium leguminosarum to legume roots at acid, neutral, and alkaline pHs |
| title_fullStr | Factors governing attachment of Rhizobium leguminosarum to legume roots at acid, neutral, and alkaline pHs |
| title_full_unstemmed | Factors governing attachment of Rhizobium leguminosarum to legume roots at acid, neutral, and alkaline pHs |
| title_short | Factors governing attachment of Rhizobium leguminosarum to legume roots at acid, neutral, and alkaline pHs |
| title_sort | factors governing attachment of rhizobium leguminosarum to legume roots at acid neutral and alkaline phs |
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