Sex-specific genetic control of Ae. aegypti and Ae. albopictus
<p>"One of the deadliest animals in the world", is the description given to the mosquito by the World Health Organisation. Collectively mosquitoes are a vector for a range of pathogens to humans. Recent developments in genetic engineering have demonstrated that the transgenic self-li...
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| Format: | Thesis |
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2016
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| _version_ | 1797107872123846656 |
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| author | Sulston, E |
| author2 | Shimeld, S |
| author_facet | Shimeld, S Sulston, E |
| author_sort | Sulston, E |
| collection | OXFORD |
| description | <p>"One of the deadliest animals in the world", is the description given to the mosquito by the World Health Organisation. Collectively mosquitoes are a vector for a range of pathogens to humans. Recent developments in genetic engineering have demonstrated that the transgenic self-limiting technology developed by Oxitec Ltd can be deployed to reduce populations of <em>Aedes aegypti</em>. The technology uses a transgene that, when inherited, causes lethality if the mosquito does not have access to tetracycline during development. Recent advancements at Oxitec Ltd involve targeting the self-limiting system specifically to females. This allows for males (which are required for a release) to be readily separated from females without the need for costly mechanical separation.</p> <p>The work presented in this thesis aims primarily to further development of the Oxitec Ltd technology in <em>Ae. aegypti</em> and <em>Aedes albopictus</em>. In <em>Ae. aegypti</em> this is achieved through the development of paternal effect, a male specific construct which render the sperm inviable if reared in the absence of tetracycline. This paternal effect is subsequently combined with a genetic sexing strain in an 'all in one' construct, such that when reared in the absence of tetracycline only sterile males are produced. Development in <em>Ae. albopictus</em> comes through characterisation of certain life history traits of the wild type strains currently maintained at Oxitec Ltd. It is important to develop self-limiting insects in a background strain with certain traits, such as the ability to be produced under mass rearing conditions, and sensitivity to insecticides. </p> <p>In conclusion the work presented here represents a major advancement of Oxitec Ltd's self-limiting technology in <em>Ae. aegypti</em>, through production of a genetic sexing strain capable of producing sterile males. In <em>Ae. albopictus</em> wild type characterisation has laid the foundations for future transgenic development in a suitable background strain.</p> |
| first_indexed | 2024-03-07T07:21:45Z |
| format | Thesis |
| id | oxford-uuid:ba501c1a-a428-4af5-83be-ae47fc2f0683 |
| institution | University of Oxford |
| last_indexed | 2024-03-07T07:21:45Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | oxford-uuid:ba501c1a-a428-4af5-83be-ae47fc2f06832022-10-11T11:14:31ZSex-specific genetic control of Ae. aegypti and Ae. albopictusThesishttp://purl.org/coar/resource_type/c_db06uuid:ba501c1a-a428-4af5-83be-ae47fc2f0683ORA Deposit2016Sulston, EShimeld, SMatzen, K<p>"One of the deadliest animals in the world", is the description given to the mosquito by the World Health Organisation. Collectively mosquitoes are a vector for a range of pathogens to humans. Recent developments in genetic engineering have demonstrated that the transgenic self-limiting technology developed by Oxitec Ltd can be deployed to reduce populations of <em>Aedes aegypti</em>. The technology uses a transgene that, when inherited, causes lethality if the mosquito does not have access to tetracycline during development. Recent advancements at Oxitec Ltd involve targeting the self-limiting system specifically to females. This allows for males (which are required for a release) to be readily separated from females without the need for costly mechanical separation.</p> <p>The work presented in this thesis aims primarily to further development of the Oxitec Ltd technology in <em>Ae. aegypti</em> and <em>Aedes albopictus</em>. In <em>Ae. aegypti</em> this is achieved through the development of paternal effect, a male specific construct which render the sperm inviable if reared in the absence of tetracycline. This paternal effect is subsequently combined with a genetic sexing strain in an 'all in one' construct, such that when reared in the absence of tetracycline only sterile males are produced. Development in <em>Ae. albopictus</em> comes through characterisation of certain life history traits of the wild type strains currently maintained at Oxitec Ltd. It is important to develop self-limiting insects in a background strain with certain traits, such as the ability to be produced under mass rearing conditions, and sensitivity to insecticides. </p> <p>In conclusion the work presented here represents a major advancement of Oxitec Ltd's self-limiting technology in <em>Ae. aegypti</em>, through production of a genetic sexing strain capable of producing sterile males. In <em>Ae. albopictus</em> wild type characterisation has laid the foundations for future transgenic development in a suitable background strain.</p> |
| spellingShingle | Sulston, E Sex-specific genetic control of Ae. aegypti and Ae. albopictus |
| title | Sex-specific genetic control of Ae. aegypti and Ae. albopictus |
| title_full | Sex-specific genetic control of Ae. aegypti and Ae. albopictus |
| title_fullStr | Sex-specific genetic control of Ae. aegypti and Ae. albopictus |
| title_full_unstemmed | Sex-specific genetic control of Ae. aegypti and Ae. albopictus |
| title_short | Sex-specific genetic control of Ae. aegypti and Ae. albopictus |
| title_sort | sex specific genetic control of ae aegypti and ae albopictus |
| work_keys_str_mv | AT sulstone sexspecificgeneticcontrolofaeaegyptiandaealbopictus |