Antisera recognising HLA-A, -B and DRW antigens raised in rhesus monkeys.
Rhesus monkeys were immunized with partially purified HLA-A, -B, -C and DR antigens. The resulting sera were shown to have activity against species-specific determinants on both HLA-A, -B, -C chains and beta 2 microglobulin by the use of somatic cell hybrids. When this was removed by absorption, the...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1980
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| _version_ | 1826293423962849280 |
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| author | Barnstable, C Snary, D Crumpton, M Balner, H Bodmer, W |
| author_facet | Barnstable, C Snary, D Crumpton, M Balner, H Bodmer, W |
| author_sort | Barnstable, C |
| collection | OXFORD |
| description | Rhesus monkeys were immunized with partially purified HLA-A, -B, -C and DR antigens. The resulting sera were shown to have activity against species-specific determinants on both HLA-A, -B, -C chains and beta 2 microglobulin by the use of somatic cell hybrids. When this was removed by absorption, the sera showed activity against three of the four HLA-A and -B antigens in the immunogen when tested on a panel of peripheral blood lymphocytes and T cells. Antibodies recognizing HLA-DR antigens were detected by testing platelet absorbed sera on a panel of typed lymphoblastoid cell lines. After absorption to remove activity against species-specific determinants on the HLA-DR antigens, two cross reacting specificities were defined. One consisted of a determinant in common between HLA-DRw1, 2 and 6 and the other a putative determinant in common between HLA-DRw4, and 5. The nature and significance of these cross-reacting groups of HLA-DR antigens is discussed in the light of current HLA-DR serology and the nature of HLA antigens in general. |
| first_indexed | 2024-03-07T03:29:52Z |
| format | Journal article |
| id | oxford-uuid:ba5815cd-2ca3-4954-970f-a29e0d9a5936 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:29:52Z |
| publishDate | 1980 |
| record_format | dspace |
| spelling | oxford-uuid:ba5815cd-2ca3-4954-970f-a29e0d9a59362022-03-27T05:09:05ZAntisera recognising HLA-A, -B and DRW antigens raised in rhesus monkeys.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ba5815cd-2ca3-4954-970f-a29e0d9a5936EnglishSymplectic Elements at Oxford1980Barnstable, CSnary, DCrumpton, MBalner, HBodmer, WRhesus monkeys were immunized with partially purified HLA-A, -B, -C and DR antigens. The resulting sera were shown to have activity against species-specific determinants on both HLA-A, -B, -C chains and beta 2 microglobulin by the use of somatic cell hybrids. When this was removed by absorption, the sera showed activity against three of the four HLA-A and -B antigens in the immunogen when tested on a panel of peripheral blood lymphocytes and T cells. Antibodies recognizing HLA-DR antigens were detected by testing platelet absorbed sera on a panel of typed lymphoblastoid cell lines. After absorption to remove activity against species-specific determinants on the HLA-DR antigens, two cross reacting specificities were defined. One consisted of a determinant in common between HLA-DRw1, 2 and 6 and the other a putative determinant in common between HLA-DRw4, and 5. The nature and significance of these cross-reacting groups of HLA-DR antigens is discussed in the light of current HLA-DR serology and the nature of HLA antigens in general. |
| spellingShingle | Barnstable, C Snary, D Crumpton, M Balner, H Bodmer, W Antisera recognising HLA-A, -B and DRW antigens raised in rhesus monkeys. |
| title | Antisera recognising HLA-A, -B and DRW antigens raised in rhesus monkeys. |
| title_full | Antisera recognising HLA-A, -B and DRW antigens raised in rhesus monkeys. |
| title_fullStr | Antisera recognising HLA-A, -B and DRW antigens raised in rhesus monkeys. |
| title_full_unstemmed | Antisera recognising HLA-A, -B and DRW antigens raised in rhesus monkeys. |
| title_short | Antisera recognising HLA-A, -B and DRW antigens raised in rhesus monkeys. |
| title_sort | antisera recognising hla a b and drw antigens raised in rhesus monkeys |
| work_keys_str_mv | AT barnstablec antiserarecognisinghlaabanddrwantigensraisedinrhesusmonkeys AT snaryd antiserarecognisinghlaabanddrwantigensraisedinrhesusmonkeys AT crumptonm antiserarecognisinghlaabanddrwantigensraisedinrhesusmonkeys AT balnerh antiserarecognisinghlaabanddrwantigensraisedinrhesusmonkeys AT bodmerw antiserarecognisinghlaabanddrwantigensraisedinrhesusmonkeys |