Combining RNA interference mutants and comparative proteomics to identify protein components and dependences in a eukaryotic flagellum.
Eukaryotic flagella from organisms such as Trypanosoma brucei can be isolated and their protein components identified by mass spectrometry. Here we used a comparative approach utilizing two-dimensional difference gel electrophoresis and isobaric tags for relative and absolute quantitation to reveal...
Κύριοι συγγραφείς: | Portman, N, Lacomble, S, Thomas, B, McKean, P, Gull, K |
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Μορφή: | Journal article |
Γλώσσα: | English |
Έκδοση: |
2009
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Παρόμοια τεκμήρια
Παρόμοια τεκμήρια
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Gamma-tubulin functions in the nucleation of a discrete subset of microtubules in the eukaryotic flagellum.
ανά: McKean, P, κ.ά.
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Swimming with protists: perception, motility and flagellum assembly.
ανά: Ginger, M, κ.ά.
Έκδοση: (2008) -
Basal body and flagellum mutants reveal a rotational constraint of the central pair microtubules in the axonemes of trypanosomes.
ανά: Gadelha, C, κ.ά.
Έκδοση: (2006) -
The eukaryotic flagellum in health and disease
ανά: Farr, H
Έκδοση: (2007) -
Flagellum ontogeny in trypanosomes studied via an inherited and regulated RNA interference system.
ανά: Bastin, P, κ.ά.
Έκδοση: (2000)