Combining RNA interference mutants and comparative proteomics to identify protein components and dependences in a eukaryotic flagellum.

Combining RNA interference mutants and comparative proteomics to identify protein components and dependences in a eukaryotic flagellum.

Eukaryotic flagella from organisms such as Trypanosoma brucei can be isolated and their protein components identified by mass spectrometry. Here we used a comparative approach utilizing two-dimensional difference gel electrophoresis and isobaric tags for relative and absolute quantitation to reveal...

书目详细资料
Main Authors:	Portman, N, Lacomble, S, Thomas, B, McKean, P, Gull, K
格式:	Journal article
语言:	English
出版:	2009

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