Clonality of cell populations in refractory anaemia using combined approach of gene loss and X-linked restriction fragment length polymorphism-methylation analyses.
We have used X-linked restriction fragment length polymorphism (RFLP)-methylation and gene deletion analyses to investigate the nature of the progenitor cell of origin in the myelodysplastic syndromes (MDS). Gene deletion studies were performed on the granulocyte and T-lymphocyte fractions of six wo...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1991
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| _version_ | 1797091244220874752 |
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| author | Abrahamson, G Boultwood, J Madden, J Kelly, S Oscier, D Rack, K Buckle, V Wainscoat, J |
| author_facet | Abrahamson, G Boultwood, J Madden, J Kelly, S Oscier, D Rack, K Buckle, V Wainscoat, J |
| author_sort | Abrahamson, G |
| collection | OXFORD |
| description | We have used X-linked restriction fragment length polymorphism (RFLP)-methylation and gene deletion analyses to investigate the nature of the progenitor cell of origin in the myelodysplastic syndromes (MDS). Gene deletion studies were performed on the granulocyte and T-lymphocyte fractions of six women with refractory anaemia (RA) and either a partial deletion of the long arm of chromosome 5 (5q-) or monosomy 7. All six showed gene loss in the granulocyte but not the T-lymphocyte fractions, indicating monoclonality of the granulocytes but not the T-lymphocytes. In order to further investigate this finding, we subsequently performed X-RFLP-methylation studies using the probe M27 beta, and also a probe for the phosphoglycerate kinase (PGK) gene. These studies have confirmed the monoclonality of the granulocytes and the polyclonality of the T-lymphocytes in these cases. Our findings suggest that in this group of patients with MDS the T-lymphocytes were not involved in the disorder, and furthermore, in the one case where B-lymphocytes were also available, that the progenitor cell of origin was restricted to the myeloid lineage. |
| first_indexed | 2024-03-07T03:30:13Z |
| format | Journal article |
| id | oxford-uuid:ba76d958-7b26-452c-8bd0-e852e2e36142 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:30:13Z |
| publishDate | 1991 |
| record_format | dspace |
| spelling | oxford-uuid:ba76d958-7b26-452c-8bd0-e852e2e361422022-03-27T05:10:02ZClonality of cell populations in refractory anaemia using combined approach of gene loss and X-linked restriction fragment length polymorphism-methylation analyses.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ba76d958-7b26-452c-8bd0-e852e2e36142EnglishSymplectic Elements at Oxford1991Abrahamson, GBoultwood, JMadden, JKelly, SOscier, DRack, KBuckle, VWainscoat, JWe have used X-linked restriction fragment length polymorphism (RFLP)-methylation and gene deletion analyses to investigate the nature of the progenitor cell of origin in the myelodysplastic syndromes (MDS). Gene deletion studies were performed on the granulocyte and T-lymphocyte fractions of six women with refractory anaemia (RA) and either a partial deletion of the long arm of chromosome 5 (5q-) or monosomy 7. All six showed gene loss in the granulocyte but not the T-lymphocyte fractions, indicating monoclonality of the granulocytes but not the T-lymphocytes. In order to further investigate this finding, we subsequently performed X-RFLP-methylation studies using the probe M27 beta, and also a probe for the phosphoglycerate kinase (PGK) gene. These studies have confirmed the monoclonality of the granulocytes and the polyclonality of the T-lymphocytes in these cases. Our findings suggest that in this group of patients with MDS the T-lymphocytes were not involved in the disorder, and furthermore, in the one case where B-lymphocytes were also available, that the progenitor cell of origin was restricted to the myeloid lineage. |
| spellingShingle | Abrahamson, G Boultwood, J Madden, J Kelly, S Oscier, D Rack, K Buckle, V Wainscoat, J Clonality of cell populations in refractory anaemia using combined approach of gene loss and X-linked restriction fragment length polymorphism-methylation analyses. |
| title | Clonality of cell populations in refractory anaemia using combined approach of gene loss and X-linked restriction fragment length polymorphism-methylation analyses. |
| title_full | Clonality of cell populations in refractory anaemia using combined approach of gene loss and X-linked restriction fragment length polymorphism-methylation analyses. |
| title_fullStr | Clonality of cell populations in refractory anaemia using combined approach of gene loss and X-linked restriction fragment length polymorphism-methylation analyses. |
| title_full_unstemmed | Clonality of cell populations in refractory anaemia using combined approach of gene loss and X-linked restriction fragment length polymorphism-methylation analyses. |
| title_short | Clonality of cell populations in refractory anaemia using combined approach of gene loss and X-linked restriction fragment length polymorphism-methylation analyses. |
| title_sort | clonality of cell populations in refractory anaemia using combined approach of gene loss and x linked restriction fragment length polymorphism methylation analyses |
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