Splenic dendritic cell subsets prime and boost CD8 T cells and are involved in the generation of effector CD8 T cells.
The ability of the dendritic cell (DC) subsets, CD8alpha+ and CD8alpha- DCs, to initiate a CD8 T cell response or to activate memory CD8 T cells and generate effector CD8 T cells has been controversial. In this study, we analyse the capacity of splenic DC subsets to induce CD8 T cell responses to a...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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2004
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| _version_ | 1826293481813835776 |
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| author | Behboudi, S Moore, A Hill, A |
| author_facet | Behboudi, S Moore, A Hill, A |
| author_sort | Behboudi, S |
| collection | OXFORD |
| description | The ability of the dendritic cell (DC) subsets, CD8alpha+ and CD8alpha- DCs, to initiate a CD8 T cell response or to activate memory CD8 T cells and generate effector CD8 T cells has been controversial. In this study, we analyse the capacity of splenic DC subsets to induce CD8 T cell responses to a CD8 T cell epitope (pb9) of a malaria antigen. The administration of peptide-pulsed CD8alpha- or CD8alpha+ DCs primes and boosts a primed CD8 T cell response against the malaria epitope. In vitro, depletion of CD11c(+) DCs from mouse splenocytes, immunised with recombinant vaccinia virus Ankara (MVA) expressing pb9 epitope, significantly reduced the generation of pb9-specific IFNgamma producing effector CD8 T cells, indicating that splenic DCs are involved in the development of pb9-specific IFNgamma producing effector cells. Taken together, this result shows that both DC subsets have the ability to prime and boost CD8 T cell responses and are involved in the activation of memory CD8 T cells. |
| first_indexed | 2024-03-07T03:30:46Z |
| format | Journal article |
| id | oxford-uuid:baa3c62c-1b20-4039-8766-546aa7ec1a25 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:30:46Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:baa3c62c-1b20-4039-8766-546aa7ec1a252022-03-27T05:11:15ZSplenic dendritic cell subsets prime and boost CD8 T cells and are involved in the generation of effector CD8 T cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:baa3c62c-1b20-4039-8766-546aa7ec1a25EnglishSymplectic Elements at Oxford2004Behboudi, SMoore, AHill, AThe ability of the dendritic cell (DC) subsets, CD8alpha+ and CD8alpha- DCs, to initiate a CD8 T cell response or to activate memory CD8 T cells and generate effector CD8 T cells has been controversial. In this study, we analyse the capacity of splenic DC subsets to induce CD8 T cell responses to a CD8 T cell epitope (pb9) of a malaria antigen. The administration of peptide-pulsed CD8alpha- or CD8alpha+ DCs primes and boosts a primed CD8 T cell response against the malaria epitope. In vitro, depletion of CD11c(+) DCs from mouse splenocytes, immunised with recombinant vaccinia virus Ankara (MVA) expressing pb9 epitope, significantly reduced the generation of pb9-specific IFNgamma producing effector CD8 T cells, indicating that splenic DCs are involved in the development of pb9-specific IFNgamma producing effector cells. Taken together, this result shows that both DC subsets have the ability to prime and boost CD8 T cell responses and are involved in the activation of memory CD8 T cells. |
| spellingShingle | Behboudi, S Moore, A Hill, A Splenic dendritic cell subsets prime and boost CD8 T cells and are involved in the generation of effector CD8 T cells. |
| title | Splenic dendritic cell subsets prime and boost CD8 T cells and are involved in the generation of effector CD8 T cells. |
| title_full | Splenic dendritic cell subsets prime and boost CD8 T cells and are involved in the generation of effector CD8 T cells. |
| title_fullStr | Splenic dendritic cell subsets prime and boost CD8 T cells and are involved in the generation of effector CD8 T cells. |
| title_full_unstemmed | Splenic dendritic cell subsets prime and boost CD8 T cells and are involved in the generation of effector CD8 T cells. |
| title_short | Splenic dendritic cell subsets prime and boost CD8 T cells and are involved in the generation of effector CD8 T cells. |
| title_sort | splenic dendritic cell subsets prime and boost cd8 t cells and are involved in the generation of effector cd8 t cells |
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