Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy
Sensorless adaptive optics is commonly used to compensate specimen-induced aberrations in high-resolution fluorescence microscopy, but requires a bespoke approach to detect aberrations in different microscopy techniques, which hinders its widespread adoption. To overcome this limitation, we propose...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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Optical Society of America
2020
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| _version_ | 1811139956269121536 |
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| author | Antonello, J Barbotin, A Chong, EZ Rittscher, J Booth, MJ |
| author_facet | Antonello, J Barbotin, A Chong, EZ Rittscher, J Booth, MJ |
| author_sort | Antonello, J |
| collection | OXFORD |
| description | Sensorless adaptive optics is commonly used to compensate specimen-induced aberrations in high-resolution fluorescence microscopy, but requires a bespoke approach to detect aberrations in different microscopy techniques, which hinders its widespread adoption. To overcome this limitation, we propose using wavelet analysis to quantify the loss of resolution due to the aberrations in microscope images. By examining the variations of the wavelet coefficients at different scales, we are able to establish a multi-valued image quality metric that can be successfully deployed in different microscopy techniques. To corroborate our arguments, we provide experimental verification of our method by performing aberration correction experiments in both confocal and STED microscopy using three different specimens. |
| first_indexed | 2024-03-07T03:31:10Z |
| format | Journal article |
| id | oxford-uuid:bac38f38-98bb-4753-886b-ab84c203b5ac |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-09-25T04:14:19Z |
| publishDate | 2020 |
| publisher | Optical Society of America |
| record_format | dspace |
| spelling | oxford-uuid:bac38f38-98bb-4753-886b-ab84c203b5ac2024-07-10T10:02:07ZMulti-scale sensorless adaptive optics: application to stimulated emission depletion microscopyJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:bac38f38-98bb-4753-886b-ab84c203b5acEnglishSymplectic ElementsOptical Society of America2020Antonello, JBarbotin, AChong, EZRittscher, JBooth, MJSensorless adaptive optics is commonly used to compensate specimen-induced aberrations in high-resolution fluorescence microscopy, but requires a bespoke approach to detect aberrations in different microscopy techniques, which hinders its widespread adoption. To overcome this limitation, we propose using wavelet analysis to quantify the loss of resolution due to the aberrations in microscope images. By examining the variations of the wavelet coefficients at different scales, we are able to establish a multi-valued image quality metric that can be successfully deployed in different microscopy techniques. To corroborate our arguments, we provide experimental verification of our method by performing aberration correction experiments in both confocal and STED microscopy using three different specimens. |
| spellingShingle | Antonello, J Barbotin, A Chong, EZ Rittscher, J Booth, MJ Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy |
| title | Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy |
| title_full | Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy |
| title_fullStr | Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy |
| title_full_unstemmed | Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy |
| title_short | Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy |
| title_sort | multi scale sensorless adaptive optics application to stimulated emission depletion microscopy |
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