Dengue virus stimulation of dendritic cells induces phosphorylation- and proteolysis-dependent signalling
<p><strong>Aims:</strong> Dengue virus (DENV) infection is commonly observed in countries with tropical climates and remains a significant health hazard. No real cure has been established for the infection to date.</p> <p><strong>Methodology and results:</stron...
| Главные авторы: | , , , |
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| Формат: | Journal article |
| Опубликовано: |
Malaysian Society for Microbiology
2018
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| _version_ | 1826293706554081280 |
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| author | Howells, A McGouran, J Kessler, B Luhn, K |
| author_facet | Howells, A McGouran, J Kessler, B Luhn, K |
| author_sort | Howells, A |
| collection | OXFORD |
| description | <p><strong>Aims:</strong> Dengue virus (DENV) infection is commonly observed in countries with tropical climates and remains a significant health hazard. No real cure has been established for the infection to date.</p> <p><strong>Methodology and results:</strong> To better understand the very early molecular events during the initial infection process, we exposed primary dendritic cells with Dengue virus and analysed proteins with increased phosphorylation signatures in the first 10 min using phospho-protein enrichment and tandem mass spectrometry analysis. Upon initial viral interaction, strong phosphorylation was observed for Endoplasmin, BiP, BID, Dok-2, GEF-H1 and Calpain-2. Reduced phosphorylation was noted for Importin-5, ERp72 and Rho-GDI. Knockdown of Calpain-2, a protease activated by calcium flux, reduced DENV infection rates of primary dendritic cells as measured by focus-forming units (FFUs).</p> <p><strong>Conclusion, significance and impact of study:</strong> We conclude that Calpain-2, BID, Importin 5 and ATP/GTPases are all active along the apoptosis pathway axis, indicating that dendritic cells commit to early signalling steps of cell death upon initial viral contact</p> |
| first_indexed | 2024-03-07T03:34:18Z |
| format | Journal article |
| id | oxford-uuid:bbc5d760-b41e-4e8d-9cd1-9c47d3062b8b |
| institution | University of Oxford |
| last_indexed | 2024-03-07T03:34:18Z |
| publishDate | 2018 |
| publisher | Malaysian Society for Microbiology |
| record_format | dspace |
| spelling | oxford-uuid:bbc5d760-b41e-4e8d-9cd1-9c47d3062b8b2022-03-27T05:19:28ZDengue virus stimulation of dendritic cells induces phosphorylation- and proteolysis-dependent signallingJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:bbc5d760-b41e-4e8d-9cd1-9c47d3062b8bSymplectic Elements at OxfordMalaysian Society for Microbiology2018Howells, AMcGouran, JKessler, BLuhn, K<p><strong>Aims:</strong> Dengue virus (DENV) infection is commonly observed in countries with tropical climates and remains a significant health hazard. No real cure has been established for the infection to date.</p> <p><strong>Methodology and results:</strong> To better understand the very early molecular events during the initial infection process, we exposed primary dendritic cells with Dengue virus and analysed proteins with increased phosphorylation signatures in the first 10 min using phospho-protein enrichment and tandem mass spectrometry analysis. Upon initial viral interaction, strong phosphorylation was observed for Endoplasmin, BiP, BID, Dok-2, GEF-H1 and Calpain-2. Reduced phosphorylation was noted for Importin-5, ERp72 and Rho-GDI. Knockdown of Calpain-2, a protease activated by calcium flux, reduced DENV infection rates of primary dendritic cells as measured by focus-forming units (FFUs).</p> <p><strong>Conclusion, significance and impact of study:</strong> We conclude that Calpain-2, BID, Importin 5 and ATP/GTPases are all active along the apoptosis pathway axis, indicating that dendritic cells commit to early signalling steps of cell death upon initial viral contact</p> |
| spellingShingle | Howells, A McGouran, J Kessler, B Luhn, K Dengue virus stimulation of dendritic cells induces phosphorylation- and proteolysis-dependent signalling |
| title | Dengue virus stimulation of dendritic cells induces phosphorylation- and proteolysis-dependent signalling |
| title_full | Dengue virus stimulation of dendritic cells induces phosphorylation- and proteolysis-dependent signalling |
| title_fullStr | Dengue virus stimulation of dendritic cells induces phosphorylation- and proteolysis-dependent signalling |
| title_full_unstemmed | Dengue virus stimulation of dendritic cells induces phosphorylation- and proteolysis-dependent signalling |
| title_short | Dengue virus stimulation of dendritic cells induces phosphorylation- and proteolysis-dependent signalling |
| title_sort | dengue virus stimulation of dendritic cells induces phosphorylation and proteolysis dependent signalling |
| work_keys_str_mv | AT howellsa denguevirusstimulationofdendriticcellsinducesphosphorylationandproteolysisdependentsignalling AT mcgouranj denguevirusstimulationofdendriticcellsinducesphosphorylationandproteolysisdependentsignalling AT kesslerb denguevirusstimulationofdendriticcellsinducesphosphorylationandproteolysisdependentsignalling AT luhnk denguevirusstimulationofdendriticcellsinducesphosphorylationandproteolysisdependentsignalling |