Multi-synaptic boutons are a feature of CA1 hippocampal connections in the stratum oriens
Excitatory synapses are typically described as single synaptic boutons (SSBs), where one presynaptic bouton contacts a single postsynaptic spine. Using serial section block-face scanning electron microscopy, we found that this textbook definition of the synapse does not fully apply to the CA1 region...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Cell Press
2023
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| _version_ | 1797112844286689280 |
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| author | Rigby, M Grillo, FW Compans, B Neves, G Gallinaro, J Nashashibi, S Horton, S Pereira Machado, PM Carbajal, MA Vizcay-Barrena, G Levet, F Sibarita, J-B Kirkland, A Fleck, RA Clopath, C Burrone, J |
| author_facet | Rigby, M Grillo, FW Compans, B Neves, G Gallinaro, J Nashashibi, S Horton, S Pereira Machado, PM Carbajal, MA Vizcay-Barrena, G Levet, F Sibarita, J-B Kirkland, A Fleck, RA Clopath, C Burrone, J |
| author_sort | Rigby, M |
| collection | OXFORD |
| description | Excitatory synapses are typically described as single synaptic boutons (SSBs), where one presynaptic bouton contacts a single postsynaptic spine. Using serial section block-face scanning electron microscopy, we found that this textbook definition of the synapse does not fully apply to the CA1 region of the hippocampus. Roughly half of all excitatory synapses in the stratum oriens involved multi-synaptic boutons (MSBs), where a single presynaptic bouton containing multiple active zones contacted many postsynaptic spines (from 2 to 7) on the basal dendrites of different cells. The fraction of MSBs increased during development (from postnatal day 22 [P22] to P100) and decreased with distance from the soma. Curiously, synaptic properties such as active zone (AZ) or postsynaptic density (PSD) size exhibited less within-MSB variation when compared with neighboring SSBs, features that were confirmed by super-resolution light microscopy. Computer simulations suggest that these properties favor synchronous activity in CA1 networks. |
| first_indexed | 2024-04-09T03:55:14Z |
| format | Journal article |
| id | oxford-uuid:bbfa8627-32ad-466a-bfca-6af471333059 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-04-09T03:55:14Z |
| publishDate | 2023 |
| publisher | Cell Press |
| record_format | dspace |
| spelling | oxford-uuid:bbfa8627-32ad-466a-bfca-6af4713330592024-03-08T12:11:42ZMulti-synaptic boutons are a feature of CA1 hippocampal connections in the stratum oriensJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:bbfa8627-32ad-466a-bfca-6af471333059EnglishSymplectic ElementsCell Press2023Rigby, MGrillo, FWCompans, BNeves, GGallinaro, JNashashibi, SHorton, SPereira Machado, PMCarbajal, MAVizcay-Barrena, GLevet, FSibarita, J-BKirkland, AFleck, RAClopath, CBurrone, JExcitatory synapses are typically described as single synaptic boutons (SSBs), where one presynaptic bouton contacts a single postsynaptic spine. Using serial section block-face scanning electron microscopy, we found that this textbook definition of the synapse does not fully apply to the CA1 region of the hippocampus. Roughly half of all excitatory synapses in the stratum oriens involved multi-synaptic boutons (MSBs), where a single presynaptic bouton containing multiple active zones contacted many postsynaptic spines (from 2 to 7) on the basal dendrites of different cells. The fraction of MSBs increased during development (from postnatal day 22 [P22] to P100) and decreased with distance from the soma. Curiously, synaptic properties such as active zone (AZ) or postsynaptic density (PSD) size exhibited less within-MSB variation when compared with neighboring SSBs, features that were confirmed by super-resolution light microscopy. Computer simulations suggest that these properties favor synchronous activity in CA1 networks. |
| spellingShingle | Rigby, M Grillo, FW Compans, B Neves, G Gallinaro, J Nashashibi, S Horton, S Pereira Machado, PM Carbajal, MA Vizcay-Barrena, G Levet, F Sibarita, J-B Kirkland, A Fleck, RA Clopath, C Burrone, J Multi-synaptic boutons are a feature of CA1 hippocampal connections in the stratum oriens |
| title | Multi-synaptic boutons are a feature of CA1 hippocampal connections in the stratum oriens |
| title_full | Multi-synaptic boutons are a feature of CA1 hippocampal connections in the stratum oriens |
| title_fullStr | Multi-synaptic boutons are a feature of CA1 hippocampal connections in the stratum oriens |
| title_full_unstemmed | Multi-synaptic boutons are a feature of CA1 hippocampal connections in the stratum oriens |
| title_short | Multi-synaptic boutons are a feature of CA1 hippocampal connections in the stratum oriens |
| title_sort | multi synaptic boutons are a feature of ca1 hippocampal connections in the stratum oriens |
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