A role for Snf2-related nucleosome-spacing enzymes in genome-wide nucleosome organization.
The positioning of nucleosomes within the coding regions of eukaryotic genes is aligned with respect to transcriptional start sites. This organization is likely to influence many genetic processes, requiring access to the underlying DNA. Here, we show that the combined action of Isw1 and Chd1 nucleo...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2011
|
| _version_ | 1797091647588139008 |
|---|---|
| author | Gkikopoulos, T Schofield, P Singh, V Pinskaya, M Mellor, J Smolle, M Workman, J Barton, G Owen-Hughes, T |
| author_facet | Gkikopoulos, T Schofield, P Singh, V Pinskaya, M Mellor, J Smolle, M Workman, J Barton, G Owen-Hughes, T |
| author_sort | Gkikopoulos, T |
| collection | OXFORD |
| description | The positioning of nucleosomes within the coding regions of eukaryotic genes is aligned with respect to transcriptional start sites. This organization is likely to influence many genetic processes, requiring access to the underlying DNA. Here, we show that the combined action of Isw1 and Chd1 nucleosome-spacing enzymes is required to maintain this organization. In the absence of these enzymes, regular positioning of the majority of nucleosomes is lost. Exceptions include the region upstream of the promoter, the +1 nucleosome, and a subset of locations distributed throughout coding regions where other factors are likely to be involved. These observations indicate that adenosine triphosphate-dependent remodeling enzymes are responsible for directing the positioning of the majority of nucleosomes within the Saccharomyces cerevisiae genome. |
| first_indexed | 2024-03-07T03:35:59Z |
| format | Journal article |
| id | oxford-uuid:bc4c7f89-eae5-4141-ba8c-4bfe19dc7624 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:35:59Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:bc4c7f89-eae5-4141-ba8c-4bfe19dc76242022-03-27T05:23:26ZA role for Snf2-related nucleosome-spacing enzymes in genome-wide nucleosome organization.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:bc4c7f89-eae5-4141-ba8c-4bfe19dc7624EnglishSymplectic Elements at Oxford2011Gkikopoulos, TSchofield, PSingh, VPinskaya, MMellor, JSmolle, MWorkman, JBarton, GOwen-Hughes, TThe positioning of nucleosomes within the coding regions of eukaryotic genes is aligned with respect to transcriptional start sites. This organization is likely to influence many genetic processes, requiring access to the underlying DNA. Here, we show that the combined action of Isw1 and Chd1 nucleosome-spacing enzymes is required to maintain this organization. In the absence of these enzymes, regular positioning of the majority of nucleosomes is lost. Exceptions include the region upstream of the promoter, the +1 nucleosome, and a subset of locations distributed throughout coding regions where other factors are likely to be involved. These observations indicate that adenosine triphosphate-dependent remodeling enzymes are responsible for directing the positioning of the majority of nucleosomes within the Saccharomyces cerevisiae genome. |
| spellingShingle | Gkikopoulos, T Schofield, P Singh, V Pinskaya, M Mellor, J Smolle, M Workman, J Barton, G Owen-Hughes, T A role for Snf2-related nucleosome-spacing enzymes in genome-wide nucleosome organization. |
| title | A role for Snf2-related nucleosome-spacing enzymes in genome-wide nucleosome organization. |
| title_full | A role for Snf2-related nucleosome-spacing enzymes in genome-wide nucleosome organization. |
| title_fullStr | A role for Snf2-related nucleosome-spacing enzymes in genome-wide nucleosome organization. |
| title_full_unstemmed | A role for Snf2-related nucleosome-spacing enzymes in genome-wide nucleosome organization. |
| title_short | A role for Snf2-related nucleosome-spacing enzymes in genome-wide nucleosome organization. |
| title_sort | role for snf2 related nucleosome spacing enzymes in genome wide nucleosome organization |
| work_keys_str_mv | AT gkikopoulost aroleforsnf2relatednucleosomespacingenzymesingenomewidenucleosomeorganization AT schofieldp aroleforsnf2relatednucleosomespacingenzymesingenomewidenucleosomeorganization AT singhv aroleforsnf2relatednucleosomespacingenzymesingenomewidenucleosomeorganization AT pinskayam aroleforsnf2relatednucleosomespacingenzymesingenomewidenucleosomeorganization AT mellorj aroleforsnf2relatednucleosomespacingenzymesingenomewidenucleosomeorganization AT smollem aroleforsnf2relatednucleosomespacingenzymesingenomewidenucleosomeorganization AT workmanj aroleforsnf2relatednucleosomespacingenzymesingenomewidenucleosomeorganization AT bartong aroleforsnf2relatednucleosomespacingenzymesingenomewidenucleosomeorganization AT owenhughest aroleforsnf2relatednucleosomespacingenzymesingenomewidenucleosomeorganization AT gkikopoulost roleforsnf2relatednucleosomespacingenzymesingenomewidenucleosomeorganization AT schofieldp roleforsnf2relatednucleosomespacingenzymesingenomewidenucleosomeorganization AT singhv roleforsnf2relatednucleosomespacingenzymesingenomewidenucleosomeorganization AT pinskayam roleforsnf2relatednucleosomespacingenzymesingenomewidenucleosomeorganization AT mellorj roleforsnf2relatednucleosomespacingenzymesingenomewidenucleosomeorganization AT smollem roleforsnf2relatednucleosomespacingenzymesingenomewidenucleosomeorganization AT workmanj roleforsnf2relatednucleosomespacingenzymesingenomewidenucleosomeorganization AT bartong roleforsnf2relatednucleosomespacingenzymesingenomewidenucleosomeorganization AT owenhughest roleforsnf2relatednucleosomespacingenzymesingenomewidenucleosomeorganization |