Antiviral immune responses in mice deficient for both interleukin-2 and interleukin-4.
Antiviral immune responses of mice lacking interleukin-2 (IL-2) or IL-4 or both IL-2 and IL-4 (IL-2/4) were compared by using different viruses. Primary cytotoxic T-lymphocyte (CTL) responses against lymphocytic choriomeningitis virus (LCMV) were only moderately reduced in mice lacking IL-2 and were...
| 主要な著者: | , , , , , , |
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| フォーマット: | Journal article |
| 言語: | English |
| 出版事項: |
1995
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| _version_ | 1826293838940995584 |
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| author | Bachmann, M Schorle, H Kühn, R Müller, W Hengartner, H Zinkernagel, R Horak, I |
| author_facet | Bachmann, M Schorle, H Kühn, R Müller, W Hengartner, H Zinkernagel, R Horak, I |
| author_sort | Bachmann, M |
| collection | OXFORD |
| description | Antiviral immune responses of mice lacking interleukin-2 (IL-2) or IL-4 or both IL-2 and IL-4 (IL-2/4) were compared by using different viruses. Primary cytotoxic T-lymphocyte (CTL) responses against lymphocytic choriomeningitis virus (LCMV) were only moderately reduced in mice lacking IL-2 and were normal in mice lacking IL-4. Mice deficient in both interleukins exhibited variable and more strongly reduced but nevertheless in vivo protective LCMV-specific CTL responses. Similar results were obtained with vaccinia virus. Upon virus-specific restimulation in vitro, spleen cells from IL-2- and IL-2/4-deficient mice failed to generate CTL responses against virus-infected target cells, whereas the response of mice deficient in only IL-4 was comparable to that of control mice. The addition of IL-2 during in vitro restimulation completely restored the responses of both IL-2 and IL-2/4-deficient mice. T-helper-cell-independent immunoglobulin M and T-helper-cell-dependent immunoglobulin G antibody responses against vesicular stomatitis virus glycoprotein were within normal ranges for the various mutant mice. After LCMV infection, specific antibody responses against LCMV nucleoprotein were reduced four- to eightfold. These results show that mice lacking IL-2/4 have an overall tendency to exhibit more severely reduced CTL responses than IL-2- or IL-4-deficient mice. Nevertheless, and surprisingly, in vivo protective immune responses were mounted in the absence of IL-2/4, suggesting that besides a minor contribution from IL-4, other interleukins compensate in vivo for the lack of IL-2 in IL-2-deficient mice. |
| first_indexed | 2024-03-07T03:36:20Z |
| format | Journal article |
| id | oxford-uuid:bc69dc34-8d8f-406d-8e4c-1ca80caa0c6c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:36:20Z |
| publishDate | 1995 |
| record_format | dspace |
| spelling | oxford-uuid:bc69dc34-8d8f-406d-8e4c-1ca80caa0c6c2022-03-27T05:24:14ZAntiviral immune responses in mice deficient for both interleukin-2 and interleukin-4.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:bc69dc34-8d8f-406d-8e4c-1ca80caa0c6cEnglishSymplectic Elements at Oxford1995Bachmann, MSchorle, HKühn, RMüller, WHengartner, HZinkernagel, RHorak, IAntiviral immune responses of mice lacking interleukin-2 (IL-2) or IL-4 or both IL-2 and IL-4 (IL-2/4) were compared by using different viruses. Primary cytotoxic T-lymphocyte (CTL) responses against lymphocytic choriomeningitis virus (LCMV) were only moderately reduced in mice lacking IL-2 and were normal in mice lacking IL-4. Mice deficient in both interleukins exhibited variable and more strongly reduced but nevertheless in vivo protective LCMV-specific CTL responses. Similar results were obtained with vaccinia virus. Upon virus-specific restimulation in vitro, spleen cells from IL-2- and IL-2/4-deficient mice failed to generate CTL responses against virus-infected target cells, whereas the response of mice deficient in only IL-4 was comparable to that of control mice. The addition of IL-2 during in vitro restimulation completely restored the responses of both IL-2 and IL-2/4-deficient mice. T-helper-cell-independent immunoglobulin M and T-helper-cell-dependent immunoglobulin G antibody responses against vesicular stomatitis virus glycoprotein were within normal ranges for the various mutant mice. After LCMV infection, specific antibody responses against LCMV nucleoprotein were reduced four- to eightfold. These results show that mice lacking IL-2/4 have an overall tendency to exhibit more severely reduced CTL responses than IL-2- or IL-4-deficient mice. Nevertheless, and surprisingly, in vivo protective immune responses were mounted in the absence of IL-2/4, suggesting that besides a minor contribution from IL-4, other interleukins compensate in vivo for the lack of IL-2 in IL-2-deficient mice. |
| spellingShingle | Bachmann, M Schorle, H Kühn, R Müller, W Hengartner, H Zinkernagel, R Horak, I Antiviral immune responses in mice deficient for both interleukin-2 and interleukin-4. |
| title | Antiviral immune responses in mice deficient for both interleukin-2 and interleukin-4. |
| title_full | Antiviral immune responses in mice deficient for both interleukin-2 and interleukin-4. |
| title_fullStr | Antiviral immune responses in mice deficient for both interleukin-2 and interleukin-4. |
| title_full_unstemmed | Antiviral immune responses in mice deficient for both interleukin-2 and interleukin-4. |
| title_short | Antiviral immune responses in mice deficient for both interleukin-2 and interleukin-4. |
| title_sort | antiviral immune responses in mice deficient for both interleukin 2 and interleukin 4 |
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