Linker histone H1.2 directs genome-wide chromatin association of the retinoblastoma tumor suppressor protein and facilitates its function
The retinoblastoma tumor suppressor protein pRb is a master regulator of cellular proliferation, principally through interaction with E2F and regulation of E2F target genes. Here, we describe the H1.2 linker histone as a major pRb interaction partner. We establish that H1.2 and pRb are found in a ch...
| Главные авторы: | , , , , , , , |
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| Формат: | Journal article |
| Язык: | English |
| Опубликовано: |
Elsevier
2017
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| _version_ | 1826293915612872704 |
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| author | Munro, S Hookway, E Floderer, M Carr, S Konietzny, R Kessler, B Oppermann, U La Thangue, N |
| author_facet | Munro, S Hookway, E Floderer, M Carr, S Konietzny, R Kessler, B Oppermann, U La Thangue, N |
| author_sort | Munro, S |
| collection | OXFORD |
| description | The retinoblastoma tumor suppressor protein pRb is a master regulator of cellular proliferation, principally through interaction with E2F and regulation of E2F target genes. Here, we describe the H1.2 linker histone as a major pRb interaction partner. We establish that H1.2 and pRb are found in a chromatin-bound complex on diverse E2F target genes. Interrogating the global influence of H1.2 on the genome-wide distribution of pRb indicated that the E2F target genes affected by H1.2 are functionally linked to cell-cycle control, consistent with the ability of H1.2 to hinder cell proliferation and the elevated levels of chromatin-bound H1-pRb complex, which occur in growth-arrested cells. Our results define a network of E2F target genes as susceptible to the regulatory influence of H1.2, where H1.2 augments global association of pRb with chromatin, enhances transcriptional repression by pRb, and facilitates pRb-dependent cell-cycle arrest. |
| first_indexed | 2024-03-07T03:37:32Z |
| format | Journal article |
| id | oxford-uuid:bccc314f-b1e3-47be-ab6a-a6b7cfbeeb79 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:37:32Z |
| publishDate | 2017 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:bccc314f-b1e3-47be-ab6a-a6b7cfbeeb792022-03-27T05:27:07ZLinker histone H1.2 directs genome-wide chromatin association of the retinoblastoma tumor suppressor protein and facilitates its functionJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:bccc314f-b1e3-47be-ab6a-a6b7cfbeeb79EnglishSymplectic Elements at OxfordElsevier2017Munro, SHookway, EFloderer, MCarr, SKonietzny, RKessler, BOppermann, ULa Thangue, NThe retinoblastoma tumor suppressor protein pRb is a master regulator of cellular proliferation, principally through interaction with E2F and regulation of E2F target genes. Here, we describe the H1.2 linker histone as a major pRb interaction partner. We establish that H1.2 and pRb are found in a chromatin-bound complex on diverse E2F target genes. Interrogating the global influence of H1.2 on the genome-wide distribution of pRb indicated that the E2F target genes affected by H1.2 are functionally linked to cell-cycle control, consistent with the ability of H1.2 to hinder cell proliferation and the elevated levels of chromatin-bound H1-pRb complex, which occur in growth-arrested cells. Our results define a network of E2F target genes as susceptible to the regulatory influence of H1.2, where H1.2 augments global association of pRb with chromatin, enhances transcriptional repression by pRb, and facilitates pRb-dependent cell-cycle arrest. |
| spellingShingle | Munro, S Hookway, E Floderer, M Carr, S Konietzny, R Kessler, B Oppermann, U La Thangue, N Linker histone H1.2 directs genome-wide chromatin association of the retinoblastoma tumor suppressor protein and facilitates its function |
| title | Linker histone H1.2 directs genome-wide chromatin association of the retinoblastoma tumor suppressor protein and facilitates its function |
| title_full | Linker histone H1.2 directs genome-wide chromatin association of the retinoblastoma tumor suppressor protein and facilitates its function |
| title_fullStr | Linker histone H1.2 directs genome-wide chromatin association of the retinoblastoma tumor suppressor protein and facilitates its function |
| title_full_unstemmed | Linker histone H1.2 directs genome-wide chromatin association of the retinoblastoma tumor suppressor protein and facilitates its function |
| title_short | Linker histone H1.2 directs genome-wide chromatin association of the retinoblastoma tumor suppressor protein and facilitates its function |
| title_sort | linker histone h1 2 directs genome wide chromatin association of the retinoblastoma tumor suppressor protein and facilitates its function |
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