The genomic basis of evolutionary innovation in Pseudomonas aeruginosa
Novel traits play a key role in evolution, but their origins remain poorly understood. Here we address this problem by using experimental evolution to study bacterial innovation in real time. We allowed 380 populations of Pseudomonas aeruginosa to adapt to 95 different carbon sources that challenged...
| Main Authors: | , , , |
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| Format: | Journal article |
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Public Library of Science
2016
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| _version_ | 1797091885015105536 |
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| author | Toll-Riera, M San Millan, A Wagner, A MacLean, R |
| author_facet | Toll-Riera, M San Millan, A Wagner, A MacLean, R |
| author_sort | Toll-Riera, M |
| collection | OXFORD |
| description | Novel traits play a key role in evolution, but their origins remain poorly understood. Here we address this problem by using experimental evolution to study bacterial innovation in real time. We allowed 380 populations of Pseudomonas aeruginosa to adapt to 95 different carbon sources that challenged bacteria with either evolving novel metabolic traits or optimizing existing traits. Whole genome sequencing of more than 80 clones revealed profound differences in the genetic basis of innovation and optimization. Innovation was associated with the rapid acquisition of mutations in genes involved in transcription and metabolism. Mutations in pre-existing duplicate genes in the P. aeruginosa genome were common during innovation, but not optimization. These duplicate genes may have been acquired by P. aeruginosa due to either spontaneous gene amplification or horizontal gene transfer. High throughput phenotype assays revealed that novelty was associated with increased pleiotropic costs that are likely to constrain innovation. However, mutations in duplicate genes with close homologs in the P. aeruginosa genome were associated with low pleiotropic costs compared to mutations in duplicate genes with distant homologs in the P. aeruginosa genome, suggesting that functional redundancy between duplicates facilitates innovation by buffering pleiotropic costs. |
| first_indexed | 2024-03-07T03:38:44Z |
| format | Journal article |
| id | oxford-uuid:bd2cf1ca-7ad2-4b41-b8a6-a15c1da5a491 |
| institution | University of Oxford |
| last_indexed | 2024-03-07T03:38:44Z |
| publishDate | 2016 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | oxford-uuid:bd2cf1ca-7ad2-4b41-b8a6-a15c1da5a4912022-03-27T05:29:51ZThe genomic basis of evolutionary innovation in Pseudomonas aeruginosaJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:bd2cf1ca-7ad2-4b41-b8a6-a15c1da5a491Symplectic Elements at OxfordPublic Library of Science2016Toll-Riera, MSan Millan, AWagner, AMacLean, RNovel traits play a key role in evolution, but their origins remain poorly understood. Here we address this problem by using experimental evolution to study bacterial innovation in real time. We allowed 380 populations of Pseudomonas aeruginosa to adapt to 95 different carbon sources that challenged bacteria with either evolving novel metabolic traits or optimizing existing traits. Whole genome sequencing of more than 80 clones revealed profound differences in the genetic basis of innovation and optimization. Innovation was associated with the rapid acquisition of mutations in genes involved in transcription and metabolism. Mutations in pre-existing duplicate genes in the P. aeruginosa genome were common during innovation, but not optimization. These duplicate genes may have been acquired by P. aeruginosa due to either spontaneous gene amplification or horizontal gene transfer. High throughput phenotype assays revealed that novelty was associated with increased pleiotropic costs that are likely to constrain innovation. However, mutations in duplicate genes with close homologs in the P. aeruginosa genome were associated with low pleiotropic costs compared to mutations in duplicate genes with distant homologs in the P. aeruginosa genome, suggesting that functional redundancy between duplicates facilitates innovation by buffering pleiotropic costs. |
| spellingShingle | Toll-Riera, M San Millan, A Wagner, A MacLean, R The genomic basis of evolutionary innovation in Pseudomonas aeruginosa |
| title | The genomic basis of evolutionary innovation in Pseudomonas aeruginosa |
| title_full | The genomic basis of evolutionary innovation in Pseudomonas aeruginosa |
| title_fullStr | The genomic basis of evolutionary innovation in Pseudomonas aeruginosa |
| title_full_unstemmed | The genomic basis of evolutionary innovation in Pseudomonas aeruginosa |
| title_short | The genomic basis of evolutionary innovation in Pseudomonas aeruginosa |
| title_sort | genomic basis of evolutionary innovation in pseudomonas aeruginosa |
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