DogCatcher allows loop-friendly protein-protein ligation

There are many efficient ways to connect proteins at termini. However, connecting at a loop is difficult because of lower flexibility and variable environment. Here we have developed DogCatcher, a protein that forms a spontaneous isopeptide bond with DogTag peptide. DogTag/DogCatcher was generated i...

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Hlavní autoři: Keeble, A, Yadav, V, Matteo, F, Bauer, C, Chuntharpursat-Bon, E, Bon, R, Huang, J, Howarth, MR
Médium: Journal article
Jazyk:English
Vydáno: Cell Press 2021