Anti-biotin antibodies offer superior organelle-specific labelling of mitochondria over avidin or streptavidin.
A number of endogenously biotinylated proteins are found in both cytosol and mitochondria of mammalian cells from many tissues, including liver, spleen, pancreas, kidney, and intestine. Therefore, caution should be taken when using the biotin detection system. Endogenous biotin can interfere with st...
| Main Authors: | , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2008
|
| _version_ | 1797092088686313472 |
|---|---|
| author | Coene, E Shaw, M Vaux, D |
| author_facet | Coene, E Shaw, M Vaux, D |
| author_sort | Coene, E |
| collection | OXFORD |
| description | A number of endogenously biotinylated proteins are found in both cytosol and mitochondria of mammalian cells from many tissues, including liver, spleen, pancreas, kidney, and intestine. Therefore, caution should be taken when using the biotin detection system. Endogenous biotin can interfere with staining systems that employ the use of biotin-avidin- or biotin-streptavidin-based detection systems and may therefore result in high, non-specific background staining. Here, we show that this endogenous biotin reactivity can be deliberately exploited and used as a specific mitochondrial marker in both light and electron microscopy as well as for identifying mitochondrial fractions on Western blot. |
| first_indexed | 2024-03-07T03:41:20Z |
| format | Journal article |
| id | oxford-uuid:bdff5a76-0226-4bf1-ad6c-35df506790a3 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:41:20Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:bdff5a76-0226-4bf1-ad6c-35df506790a32022-03-27T05:36:06ZAnti-biotin antibodies offer superior organelle-specific labelling of mitochondria over avidin or streptavidin.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:bdff5a76-0226-4bf1-ad6c-35df506790a3EnglishSymplectic Elements at Oxford2008Coene, EShaw, MVaux, DA number of endogenously biotinylated proteins are found in both cytosol and mitochondria of mammalian cells from many tissues, including liver, spleen, pancreas, kidney, and intestine. Therefore, caution should be taken when using the biotin detection system. Endogenous biotin can interfere with staining systems that employ the use of biotin-avidin- or biotin-streptavidin-based detection systems and may therefore result in high, non-specific background staining. Here, we show that this endogenous biotin reactivity can be deliberately exploited and used as a specific mitochondrial marker in both light and electron microscopy as well as for identifying mitochondrial fractions on Western blot. |
| spellingShingle | Coene, E Shaw, M Vaux, D Anti-biotin antibodies offer superior organelle-specific labelling of mitochondria over avidin or streptavidin. |
| title | Anti-biotin antibodies offer superior organelle-specific labelling of mitochondria over avidin or streptavidin. |
| title_full | Anti-biotin antibodies offer superior organelle-specific labelling of mitochondria over avidin or streptavidin. |
| title_fullStr | Anti-biotin antibodies offer superior organelle-specific labelling of mitochondria over avidin or streptavidin. |
| title_full_unstemmed | Anti-biotin antibodies offer superior organelle-specific labelling of mitochondria over avidin or streptavidin. |
| title_short | Anti-biotin antibodies offer superior organelle-specific labelling of mitochondria over avidin or streptavidin. |
| title_sort | anti biotin antibodies offer superior organelle specific labelling of mitochondria over avidin or streptavidin |
| work_keys_str_mv | AT coenee antibiotinantibodiesoffersuperiororganellespecificlabellingofmitochondriaoveravidinorstreptavidin AT shawm antibiotinantibodiesoffersuperiororganellespecificlabellingofmitochondriaoveravidinorstreptavidin AT vauxd antibiotinantibodiesoffersuperiororganellespecificlabellingofmitochondriaoveravidinorstreptavidin |