Complete nucleotide sequence of a unique HLA class I C locus product expressed on the human choriocarcinoma cell line BeWo.
We have used Northern blot analysis to detect mRNA from class I HLA genes in the human choriocarcinoma cell line BeWo, which has been previously shown to express an atypical HLA class I molecule, in the absence of HLA A and B. Hybridization was seen with three class I DNA probes, the strongest being...
| Päätekijät: | , , , |
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| Aineistotyyppi: | Journal article |
| Kieli: | English |
| Julkaistu: |
1989
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| _version_ | 1826294183362560000 |
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| author | Ellis, SA Strachan, T Palmer, MS Mcmichael, A |
| author_facet | Ellis, SA Strachan, T Palmer, MS Mcmichael, A |
| author_sort | Ellis, SA |
| collection | OXFORD |
| description | We have used Northern blot analysis to detect mRNA from class I HLA genes in the human choriocarcinoma cell line BeWo, which has been previously shown to express an atypical HLA class I molecule, in the absence of HLA A and B. Hybridization was seen with three class I DNA probes, the strongest being seen with the probe pC800, which corresponds to an 800-bp section of the Cw3 gene. We have made cDNA libraries from BeWo cells and screened for positive clones by using class I DNA probes. Of the clones isolated, we determined the complete sequence of one and partial sequence of five shorter clones. They all code for an identical C locus-related product, which does not correspond to published C locus sequences. |
| first_indexed | 2024-03-07T03:41:42Z |
| format | Journal article |
| id | oxford-uuid:be1e5ac9-cdf4-4a2b-a3d3-b8f142630bed |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:41:42Z |
| publishDate | 1989 |
| record_format | dspace |
| spelling | oxford-uuid:be1e5ac9-cdf4-4a2b-a3d3-b8f142630bed2022-03-27T05:36:51ZComplete nucleotide sequence of a unique HLA class I C locus product expressed on the human choriocarcinoma cell line BeWo.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:be1e5ac9-cdf4-4a2b-a3d3-b8f142630bedEnglishSymplectic Elements at Oxford1989Ellis, SAStrachan, TPalmer, MSMcmichael, AWe have used Northern blot analysis to detect mRNA from class I HLA genes in the human choriocarcinoma cell line BeWo, which has been previously shown to express an atypical HLA class I molecule, in the absence of HLA A and B. Hybridization was seen with three class I DNA probes, the strongest being seen with the probe pC800, which corresponds to an 800-bp section of the Cw3 gene. We have made cDNA libraries from BeWo cells and screened for positive clones by using class I DNA probes. Of the clones isolated, we determined the complete sequence of one and partial sequence of five shorter clones. They all code for an identical C locus-related product, which does not correspond to published C locus sequences. |
| spellingShingle | Ellis, SA Strachan, T Palmer, MS Mcmichael, A Complete nucleotide sequence of a unique HLA class I C locus product expressed on the human choriocarcinoma cell line BeWo. |
| title | Complete nucleotide sequence of a unique HLA class I C locus product expressed on the human choriocarcinoma cell line BeWo. |
| title_full | Complete nucleotide sequence of a unique HLA class I C locus product expressed on the human choriocarcinoma cell line BeWo. |
| title_fullStr | Complete nucleotide sequence of a unique HLA class I C locus product expressed on the human choriocarcinoma cell line BeWo. |
| title_full_unstemmed | Complete nucleotide sequence of a unique HLA class I C locus product expressed on the human choriocarcinoma cell line BeWo. |
| title_short | Complete nucleotide sequence of a unique HLA class I C locus product expressed on the human choriocarcinoma cell line BeWo. |
| title_sort | complete nucleotide sequence of a unique hla class i c locus product expressed on the human choriocarcinoma cell line bewo |
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