Atomically resolved imaging of the conformations and adsorption geometries of individual β-cyclodextrins with non-contact AFM
Glycans, consisting of covalently linked sugar units, are a major class of biopolymers essential to all known living organisms. To better understand their biological functions and further applications in fields from biomedicine to materials science, detailed knowledge of their structure is essential...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Nature Research
2024
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| _version_ | 1817931354216595456 |
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| author | Grabarics, M Mallada, B Edalatmanesh, S Jiménez-Martín, A Pykal, M Ondráček, M Kührová, P Struwe, WB Banáš, P Rauschenbach, S Jelínek, P de la Torre, B |
| author_facet | Grabarics, M Mallada, B Edalatmanesh, S Jiménez-Martín, A Pykal, M Ondráček, M Kührová, P Struwe, WB Banáš, P Rauschenbach, S Jelínek, P de la Torre, B |
| author_sort | Grabarics, M |
| collection | OXFORD |
| description | Glycans, consisting of covalently linked sugar units, are a major class of biopolymers essential to all known living organisms. To better understand their biological functions and further applications in fields from biomedicine to materials science, detailed knowledge of their structure is essential. However, due to the extraordinary complexity and conformational flexibility of glycans, state-of-the-art glycan analysis methods often fail to provide structural information with atomic precision. Here, we combine electrospray deposition in ultra-high vacuum with non-contact atomic force microscopy and theoretical calculations to unravel the structure of β-cyclodextrin, a cyclic glucose oligomer, with atomic-scale detail. Our results, established on the single-molecule level, reveal the different adsorption geometries and conformations of β-cyclodextrin. The position of individual hydroxy groups and the location of the stabilizing intramolecular H-bonds are deduced from atomically resolved images, enabling the unambiguous assignment of the molecular structure and demonstrating the potential of the method for glycan analysis. |
| first_indexed | 2024-12-09T03:20:41Z |
| format | Journal article |
| id | oxford-uuid:be4d2600-6aee-49ab-a606-45c750a4f1b7 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-12-09T03:20:41Z |
| publishDate | 2024 |
| publisher | Nature Research |
| record_format | dspace |
| spelling | oxford-uuid:be4d2600-6aee-49ab-a606-45c750a4f1b72024-11-11T20:03:33ZAtomically resolved imaging of the conformations and adsorption geometries of individual β-cyclodextrins with non-contact AFMJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:be4d2600-6aee-49ab-a606-45c750a4f1b7EnglishJisc Publications RouterNature Research2024Grabarics, MMallada, BEdalatmanesh, SJiménez-Martín, APykal, MOndráček, MKührová, PStruwe, WBBanáš, PRauschenbach, SJelínek, Pde la Torre, BGlycans, consisting of covalently linked sugar units, are a major class of biopolymers essential to all known living organisms. To better understand their biological functions and further applications in fields from biomedicine to materials science, detailed knowledge of their structure is essential. However, due to the extraordinary complexity and conformational flexibility of glycans, state-of-the-art glycan analysis methods often fail to provide structural information with atomic precision. Here, we combine electrospray deposition in ultra-high vacuum with non-contact atomic force microscopy and theoretical calculations to unravel the structure of β-cyclodextrin, a cyclic glucose oligomer, with atomic-scale detail. Our results, established on the single-molecule level, reveal the different adsorption geometries and conformations of β-cyclodextrin. The position of individual hydroxy groups and the location of the stabilizing intramolecular H-bonds are deduced from atomically resolved images, enabling the unambiguous assignment of the molecular structure and demonstrating the potential of the method for glycan analysis. |
| spellingShingle | Grabarics, M Mallada, B Edalatmanesh, S Jiménez-Martín, A Pykal, M Ondráček, M Kührová, P Struwe, WB Banáš, P Rauschenbach, S Jelínek, P de la Torre, B Atomically resolved imaging of the conformations and adsorption geometries of individual β-cyclodextrins with non-contact AFM |
| title | Atomically resolved imaging of the conformations and adsorption geometries of individual β-cyclodextrins with non-contact AFM |
| title_full | Atomically resolved imaging of the conformations and adsorption geometries of individual β-cyclodextrins with non-contact AFM |
| title_fullStr | Atomically resolved imaging of the conformations and adsorption geometries of individual β-cyclodextrins with non-contact AFM |
| title_full_unstemmed | Atomically resolved imaging of the conformations and adsorption geometries of individual β-cyclodextrins with non-contact AFM |
| title_short | Atomically resolved imaging of the conformations and adsorption geometries of individual β-cyclodextrins with non-contact AFM |
| title_sort | atomically resolved imaging of the conformations and adsorption geometries of individual β cyclodextrins with non contact afm |
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